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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Gardeniers, Han
University of Twente
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (26/26 displayed)
- 2024Alternative nano-lithographic tools for shell-isolated nanoparticle enhanced Raman spectroscopy substrates
- 2024Alternative nano-lithographic tools for shell-isolated nanoparticle enhanced Raman spectroscopy substratescitations
- 2023Fabrication of homogeneous shell-isolated sers substrates for catalytic applications
- 20233D‐Architected Alkaline‐Earth Perovskitescitations
- 2022Fabrication of microstructures in the bulk and on the surface of sapphire by anisotropic selective wet etching of laser-affected volumescitations
- 2022Additive Manufacturing of 3D Luminescent ZrO2:Eu3+ Architecturescitations
- 2022Vacuum-driven assembly of electrostatically levitated microspheres on perforated surfacescitations
- 2020Massive Parallel NEMS Flow Restriction Fabricated Using Self-Aligned 3D-Crystallographic Nanolithographycitations
- 2020Fabrication of millimeter-long structures in sapphire using femtosecond infrared laser pulses and selective etchingcitations
- 2020Spatial Segregation of Microspheres by Rubbing-Induced Triboelectrification on Patterned Surfacescitations
- 2018Three-dimensional fractal geometry for gas permeation in microchannelscitations
- 2018Morphology of single picosecond pulse subsurface laser-induced modifications of sapphire and subsequent selective etchingcitations
- 2012Production and characterization of micro- and nano-features in biomedical alumina and zirconia ceramics using a tape casting routecitations
- 2008On the resilience of PDMS microchannels after violent optical breakdown microbubble cavitation
- 2007Integrated electrochemical sensor array for on-line monitoring of yeast fermentationscitations
- 2007Spreading of thin-film metal patterns deposited on nonplanar surfaces using a shadow mask micromachined in si (110)citations
- 2006Fabrication of microfluidic networks with integrated electrodescitations
- 2006Monitoring of yeast cell concentration using a micromachnined impedance sensorcitations
- 2005Monitoring of yeast cell concentration using a micromachined impedance sensor
- 2003A low hydraulic capacitance pressure sensor for integration with a micro viscosity detectorcitations
- 2002Fabrication and characterization of MEMS based wafer-scale palladium-silver alloy membranes for hydrogen separation and hydrogenation/dehydrogenation reactionscitations
- 2002Integrated Micro- and Nanofluidics: Silicon Revisitedcitations
- 2002Micromachined Palladium - Silver Alloy Membranes for Hydrogen Separation
- 2001Local anodic bonding of Kovar to Pyrex aimed at high-pressure, solvent-resistant microfluidic connectionscitations
- 2001Failure mechanisms of pressurized microchannels, model, and experimentscitations
- 2000Failure mechanisms of pressurized microchannels, model and experiments
Places of action
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article
Monitoring of yeast cell concentration using a micromachnined impedance sensor
Abstract
This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved