| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Del Valle, Manel
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (37/37 displayed)
- 2021Wavelet neural networks
- 2020Improved Sensing of Capsaicin with TiO2 Nanoparticles Modified Epoxy Graphite Electrode
- 2018Bioelectronic tongue using MIP sensors for the resolution of volatile phenolic compounds
- 2018Multivariate Determination of Total Sugar Content and Ethanol in Bioethanol Production Using Carbon Electrodes Modified with MWCNT/MeOOH and Chemometric Data Treatment
- 2018Label-free aptasensor for lysozyme detection using electrochemical impedance spectroscopycitations
- 2017Molecularly imprinted polymers for TNT analogues :citations
- 2017Graphene electrode platform for impedimetric aptasensing
- 2016Analysis of Amino Acid Mixtures by Voltammetric Electronic Tongues and Artificial Neural Networks
- 2016Simultaneous Voltammetric Determination of Heavy Metals by Use of Crown Ether-modified Electrodes and Chemometricscitations
- 2016Resolution of galactose, glucose, xylose and mannose in sugarcane bagasse employing a voltammetric electronic tongue formed by metals oxy-hydroxide/MWCNT modified electrodescitations
- 2015Crown ether-modified electrodes for the simultaneous stripping voltammetric determination of Cd(II), Pb(II) and Cu(II)citations
- 2015DNA polymorphism sensitive impedimetric detection on gold-nanoislands modified electrodescitations
- 2014Discrimination of Soils and Assessment of Soil Fertility Using Information from an Ion Selective Electrodes Array and Artificial Neural Networkscitations
- 2014Label-free impedimetric aptasensor based on epoxy-graphite electrode for the recognition of cytochrome c
- 2014A comparison of four protocols for the immobilization of an aptamer on graphite composite electrodes
- 2014Array of peptide-modified electrodes for the simultaneous determination of Pb(II), Cd(II) and Zn(II)
- 2014Pd nanoparticles/multiwalled carbon nanotubes electrode system for voltammetric sensing of tyrosinecitations
- 2014Impedimetric DNA Biosensors Based on Nanomaterialscitations
- 2012Determination of total polyphenol index in wines employing a voltammetric electronic tonguecitations
- 2012Impedimetric DNA Sensing Employing Nanomaterialscitations
- 2012A reusable impedimetric aptasensor for detection of thrombin employing a graphite-epoxy composite electrodecitations
- 2011Multiway processing of data generated with a potentiometric electronic tongue in a SIA systemcitations
- 2011A new amperometric bienzymatic biosensor based on biocomposites for the determination of gluconic acid in winescitations
- 2010Use of Sequential Injection Analysis to construct a potentiometric electronic tongue: Application to the multidetermination of heavy metalscitations
- 2009Electrochemical immunosensor for the diagnosis of celiac diseasecitations
- 2009Resolution of heavy metal mixtures from highly overlapped ASV voltammograms employing a wavelet neural networkcitations
- 2007EIS multianalyte sensing with an automated SIA system-An electronic tongue employing the impedimetric signalcitations
- 2007Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensorscitations
- 2007Multicomponent titration of calcium + magnesium mixtures employing a potentiometric electronic-tonguecitations
- 2006Impedimetric genosensors for the detection of DNA hybridizationcitations
- 2006Genomagnetic assay based on label-free electrochemical detection using magneto-composite electrodescitations
- 2006Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodescitations
- 2005Integration of a glucose biosensor based on an epoxy-graphite- TTF·TCNQ-GOD biocomposite into a FIA systemcitations
- 2005Glucose biosensor based on carbon nanotube epoxy compositescitations
- 2005Sensitive stripping voltammetry of heavy metals by using a composite sensor based on a built-in bismuth precursorcitations
- 2004Renewable Protein A modified graphite-epoxy composite for electrochemical immunosensingcitations
- 2004Rigid carbon composites: A new transducing material for label-free electrochemical genosensingcitations
Places of action
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article
Genomagnetic assay based on label-free electrochemical detection using magneto-composite electrodes
Abstract
A very sensitive genomagnetic assay based on a label-free electrochemical detection of the DNA target by using graphite-epoxy composite (GEC) and magneto-GEC electrodes as electrochemical transducers has been developed. The assay is based on the capture of DNA target on streptavidin magnetic beads by its hybridization with biotinylated inosine-substituted probe and its electrochemical detection achieved by the measurement of the signal coming from the guanine oxidation of the DNA target. The genomagnetic assay involves the following steps: the immobilization of biotinylated inosine-substituted capture probe onto streptavidin coated magnetic beads following by hybridization with its target DNA or in the presence of non-complementary (NC) DNA. Two different genomagnetic strategies have been developed for the selective electrochemical detection of DNA target. Firstly, an alkaline treatment was performed in order to dissociate DNA hybrids from the magnetic beads, followed by the voltammetric measurement of guanine oxidation signal using GEC electrode as an electrochemical transducer. In order to simplify the genomagnetic procedure, in the second strategy the magnetic separation was directly achieved by using a novel magneto-electrochemical transducer based on GEC electrode containing a small magnet (m-GEC). The genomagnetic assay based on label-free electrochemical detection was developed for the specific detection of a sequence related with Salmonella spp. with a target concentration of 20 μg/mL in 20 min of hybridization time. The main features related with these genomagnetic assays, such as detection limit and the reproducibility, are discussed and compared with other genomagnetic assays. © 2005 Elsevier B.V. All rights reserved.