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Richter Gomes Da Silva, Marco Diogo
in Cooperation with on an Cooperation-Score of 37%
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Publications (4/4 displayed)
- 2022Purification of ferulic acid from corn fibre alkaline extracts for bio-vanillin production using an adsorption processcitations
- 2022Antioxidant and Antibacterial Activities of a Purified Polysaccharide Extracted from Ceratonia siliqua L. and Its Involvement in the Enhancement Performance of Whipped Creamcitations
- 2017Stabilizing Unstable Amorphous Menthol through Inclusion in Mesoporous Silica Hostscitations
- 2010Electrokinetic removal of creosote from treated timber waste: a comprehensive gas chromatographic viewcitations
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article
Purification of ferulic acid from corn fibre alkaline extracts for bio-vanillin production using an adsorption process
Abstract
<p>Ferulic acid is the most widely studied precursor for bio-vanillin production. This work assesses the use of an alkaline extract from corn fibre for bio-vanillin production. The results show that after extraction an additional step is needed to purify ferulic acid removing toxic/inhibitor compounds. An adsorption process was selected to purify the ferulic acid. The performances of four different macroporous resins prepared from different matrix materials were evaluated. Macronet® MN102 (not yet reported for ferulic acid purification) offered the highest ferulic acid adsorption capacity. A column packed with Macronet® MN102 was used to perform dynamic adsorption and desorption experiments, which showed a maximum ferulic acid adsorption capacity of 176 mg<sub>ferulic acid</sub>.g<sup>−1</sup><sub>dry resin</sub> at pH 4.5 at a flow rate of 3.7 BV (bed volumes).h<sup>−1</sup>. The breakthrough point was at 115 min, corresponding to an adsorption capacity of 85 mg<sub>ferulic acid</sub>.g<sup>−1</sup><sub>dry resin</sub>. In the desorption step, 90.9% of ferulic acid was recovered using absolute ethanol ≥ 99.8%) as eluent, at the same flow rate of 3.7 BV (bed volumes).h<sup>−1</sup>. This procedure confirmed the removal of compounds with a microbial inhibitory effect, such as organic acids, metals and some aldehydes. The purified ferulic acid extract was then used to produce bio-vanillin. The bio-vanillin production by Amycolatopsis sp. ATCC 39116, using a single pulse of an extract with 10 g.L<sup>-1</sup> of ferulic acid extract, led to a maximum vanillin concentration of 5 g.L<sup>-1</sup> and a vanillin yield of 0.52 g<sub>vanillin</sub>.g<sub>ferulic acid</sub><sup>-1</sup>, values comparable to those obtained with a commercial solution containing 10 g.L<sup>-1</sup> of ferulic acid.</p>