| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Dawson, Jonathan
University of Southampton
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2023Branched copolymer surfactants impart thermoreversible gelation to LAPONITE® gels †
- 2023Branched copolymer surfactants impart thermoreversible gelation to LAPONITE® gels
- 2022Determination of protoplast growth properties using quantitative single-cell tracking analysiscitations
- 2021Nanocomposite clay-based bioinks for skeletal tissue engineeringcitations
- 2020Growth‐factor free multicomponent nanocomposite hydrogels that stimulate bone formationcitations
- 2020Bisphosphonate nanoclay edge-site interactions facilitate hydrogel self-assembly and sustained growth factor localizationcitations
- 2020Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponitecitations
- 2020Nanoclay-polyamine composite hydrogel for topical delivery of nitric oxide gas via innate gelation characteristics of laponitecitations
- 2020Nanoclay-based 3D printed scaffolds promote vascular ingrowth ex vivo and generate bone mineral tissue in vitro and in vivo
- 2019Printing bone in a gel: using nanocomposite bioink to print functionalised bone scaffoldscitations
- 2019Osteogenic and angiogenic tissue formation in high fidelity nanocomposite Laponite-gelatin bioinkscitations
- 2018Clay nanoparticles for regenerative medicine and biomaterial designcitations
- 2013A tissue engineering strategy for the treatment of avascular necrosis of the femoral headcitations
Places of action
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article
Printing bone in a gel: using nanocomposite bioink to print functionalised bone scaffolds
Abstract
Free-form printing offers a novel biofabrication approach to generate complex shapes by depositing hydrogel materials within a temporary supportive environment. However, printed hydrogels typically lack the requisite mechanical properties and functionality of the desired tissue, limiting application and, more importantly, safety and efficacy of the implant. We have developed an innovative nanoclay-based bioink to print high shape fidelity functional constructs for potential skeletal application. Laponite® (LAP) nanoclay was combined with gellan gum (GG) to generate a printable hydrogel that was highly stable in vitro, displayed limited swelling ability compared to the silicate-free control and remained stable over time. An agarose fluid gel was found to provide the requisite support for the deposition of the material ink and preservation of the printed structure prior to crosslinking. Printed C2C12 myoblasts remained viable and displayed extensive proliferation over 21 days in culture. Cell-laden scaffolds demonstrated functionality within 1 day of culture in vitro and that was preserved over 3 weeks. Analysis of absorption and release mechanisms from LAP-GG using model proteins (lysozyme and bovine serum albumin (BSA)) demonstrated the retention capability of the clay-based materials for compound localisation and absence of burst release. Vascular endothelial growth factor (VEGF) was loaded within the agarose fluid gel and absorbed by the material ink via absorbtion during deposition. The 3D printed constructs was implanted on the chorioallantoic membrane of a 10-days old developing chick. Extensive and preferential vasculature infiltration was observed in LAP-GG loaded VEGF constructs compared to controls (p<0.01 and p<0.0001) after only 7 days of incubation. The current studies demonstrate, for the first time, the application of innovative LAP-GG 3D constructs in the generation of growth factor loaded 3D constructs for potential application in skeletal tissue repair.