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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Korri-Youssoufi, Hafsa
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (16/16 displayed)
- 2024Bio-Based Materials for Electrochemical Detection of Bisphenol A
- 2024Impedimetric Detection of Cancer Markers Based on Nanofiber Copolymerscitations
- 2023Label free electrochemical sensors for Pb(II) detection based on hemicellulose extracted from Opuntia Ficus Indica cactus ; Capteurs électrochimiques sans marquage pour la détection du Pb(II) à base d'hémicellulose extraite du cactus Opuntia Ficus Indicacitations
- 2022Review—Recent Progress in Graphene Based Modified Electrodes for Electrochemical Detection of Dopaminecitations
- 2021MoS2/PPy Nanocomposite as a Transducer for Electrochemical Aptasensor of Ampicillin in River Water
- 2021MoS2/PPy Nanocomposite as a Transducer for Electrochemical Aptasensor of Ampicillin in River Watercitations
- 2020Nanocomposite Based on Poly (para-phenylene)/Chemical Reduced Graphene Oxide as a Platform for Simultaneous Detection of Ascorbic Acid, Dopamine and Uric Acidcitations
- 2020Sensor based on redox conjugated poly(para-phenylene) for the simultaneous detection of dopamine, ascorbic acid, and uric acid in human serum sample.citations
- 2020Dispersible Conjugated Polymer Nanoparticles as Biointerface Materials for Label-Free Bacteria Detectioncitations
- 2020Structural and electrochemical studies of functionalization of reduced graphene oxide with alkoxyphenylporphyrin mono- and tetra- carboxylic acid: application to DNA sensorscitations
- 2020E-DNA biosensors of M. tuberculosis based on nanostructured polypyrrolecitations
- 2019E-DNA biosensors of M. tuberculosis based on nanostructured polypyrrole.citations
- 2019E-DNA detection of rpoB gene resistance in Mycobacterium tuberculosis in real samples using Fe3O4/polypyrrole nanocompositecitations
- 2018Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs)citations
- 2015E-DNA Sensor of Mycobacterium tuberculosis Based on Electrochemical Assembly of Nanomaterials (MWCNTs/PPy/PAMAM).
- 2010Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrolecitations
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article
E-DNA biosensors of M. tuberculosis based on nanostructured polypyrrole.
Abstract
The objective of this paper is to demonstrate the potential of nanostructured polypyrrole formed by template free as platform for amperometric detection of DNA. The nanowires of polypyrrole (nw-PPy) are formed through electrochemical polymerization and chemically modified by electrochemical oxidation of ethylene diamine or dendrimers PAMAM to obtain aminated surface. The DNA probe and ferrocenyl group, as redox reporter, were covalently linked to the surface of nw-PPy. The chemical structure of nanostructured platform was characterized through SEM, FT-IR and XPS and the electrochemical properties through cyclic voltammetry and electrochemical impedance spectroscopy (EIS). We show that the properties of nw-PPy such as, hydrophilic character and large surface area have large effect on the electronic properties. Thus, the electrochemical performance is increased compared to others nanomaterials considering the obtained value of the rate of electron transfer of 18 s-1. These properties allow enhanced DNA sensing where detection limit of 0.36 atomolar without any amplification step. The biosensor can be applied in detection of genomic DNA of Mycobacterium tuberculosis and the mutated one which present the resistance to rifampicin and large selectivity was demonstrated. We believe that nw-PPy modified with redox marker is a promising platform for electrochemical biosensors and can be applied for various diagnosis prospects.