| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Mayer, Philipp
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2024Determining ecotoxicity drivers and biodegradation kinetics of discharged chemicals in produced water from oil and gas extraction in the North Sea
- 2018Assessing PCB pollution in the Baltic Sea - An equilibrium partitioning based studycitations
- 2018Headspace passive dosing for dose-response testing of volatile hydrophobic organic chemicals
- 2015Comparison of passive and standard dosing of polycyclic aromatic hydrocarbons to the marine algae Phaeodactylum tricornutum
- 2015Equilibrium passive sampling as a tool to study polycyclic aromatic hydrocarbons in Baltic Sea sediment pore-water systemscitations
- 2014The effect of humic acids on biodegradation of polycyclic aromatic hydrocarbons depends on the exposure regimecitations
- 2013Baseline Toxic Mixtures of Non-Toxic Chemicalscitations
- 2013The dosing determines mutagenicity of hydrophobic compounds in the Ames II assay with metabolic transformationcitations
- 2012Recreating the seawater mixture composition of HOCs in toxicity tests with Artemia franciscana by passive dosingcitations
- 2011A Contaminant Trap as a Tool for Isolating and Measuring the Desorption Resistant Fraction of Soil Pollutantscitations
- 2011Application of passive dosing to study the biotransformation and biodegradation of hydrophobic compounds
- 2011Application of passive dosing to study the biotransformation and biodegradation of hydrophobic
- 2010Controlling and maintaining exposure of hydrophobic organic compounds in aquatic toxicity tests by passive dosingcitations
- 2010Passive Dosing for Producing Defined and Constant Exposure of Hydrophobic Organic Compounds during in Vitro Toxicity Testscitations
- 2009In Situ Silicone Tube Microextractioncitations
Places of action
| Organizations | Location | People |
|---|
article
The dosing determines mutagenicity of hydrophobic compounds in the Ames II assay with metabolic transformation
Abstract
The Ames II bacterial mutagenicity assay is a new version of the standard Ames test for screening chemicals for genotoxic activity. However, the use of plastic micro-titer plates has drawbacks in the case of testing hydrophobic mutagens, since sorptive and other losses make it difficult to control and define the exposure concentrations, and they reduce availability for bacterial uptake or to the S9 enzymes. With passive dosing, a biocompatible polymer such as silicone is loaded with the test compound and acts as a partitioning source. It compensates for any losses and results in stable freely dissolved concentrations. Passive dosing using silicone O-rings was applied in the Ames 11 assay to measure PAH mutagenicity in strains TA98 and TAMix - a mixture of six different bacterial strains detecting six different base-pair substitutions - after metabolic activation by S9. Initially, 10 PAHs were tested with passive dosing from saturated O-rings, aiming at levels in the test medium close to aqueous solubility. Fluoranthene, pyrene and benzo(a)pyrene were mutagenic in both TA98 and TAMix, whereas benz(a)anthracene was mutagenic in TA98 only. The concentration-dependent mutagenic activity of benzo(a)pyrene was then compared for passive dosing and solvent spiking. With spiking, nominal concentrations greatly exceeded aqueous solubility before mutagenicity was observed, due to sorptive losses and limiting dissolution kinetics. In contrast, the passive dosing concentration-response curves were more reproducible, and shifted towards lower concentrations by several orders of magnitude. This study raises fundamental questions about how to introduce hydrophobic test substances in the Ames 11 assay with biotransformation, since the measured mutagenicity not only depends on the compound potency but also on its supply, sorption and consumption during the assay. (C) 2012 Elsevier B.V. All rights reserved.