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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Youseffi, Mansour
University of Bradford
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (10/10 displayed)
- 2022Tensile, rheological and morphological characterizations of multi-walled carbon nanotube/polypropylene composites prepared by microinjection and compression moldingcitations
- 2019Effect of screw configuration on the dispersion and properties of polypropylene/multiwalled carbon nanotube compositecitations
- 2015Comparison of two different indentation techniques in studying the in-situ viscoelasticity behavior of liquid crystals
- 2013Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals.citations
- 2006Sintering and mechanical properties of prealloyed 6061 Al alloy with and without common lubricants and sintering aids
- 2006Selective laser melting of prealloyed high alloy steel powder bedscitations
- 2006PM processing of elemental and prealloyed 6061 aluminium alloy with and without common lubricants and sintering aids.citations
- 2002Effects of silicon addition and process conditions on ¿-phase sintering, sinter hardening, and
- 2002Enhanced sintering and mechanical properties of 316L stainless steel with silicon additions sintering aidcitations
- 2000Sintering microstructure and mechanical properties of PM manganese-molybdenum steelscitations
Places of action
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article
Biophysical characteristics of cells cultured on cholesteryl ester liquid crystals.
Abstract
This study aimed at examining the biophysical characteristics of human derived keratinocytes (HaCaT) cultured on cholesteryl ester liquid crystals (CELC). CELC was previously shown to improve sensitivity in sensing cell contractions. Characteristics of the cell integrin expressions and presence of extracellular matrix (ECM) proteins on the liquid crystals were interrogated using various immunocytochemical techniques. The investigation was followed by characterization of the chemical properties of the liquid crystals (LC) after immersion in cell culture media using Fourier transform infrared spectroscopy (FTIR). The surface morphology of cells adhered to the LC was studied using atomic force microscopy (AFM). Consistent with the expressions of the integrins α2, α3 and β1, extracellular matrix proteins (laminin, collagen type IV and fibronectin) were found secreted by the HaCaT onto CELC and these proteins were also secreted by cells cultured on the glass substrates. FTIR analysis of the LC revealed the existence of spectrum assigned to cholesterol and ester moieties that are essential compounds for the metabolizing activities of keratinocytes. The immunostainings indicated that cell adhesion on the LC is mediated by self-secreted ECM proteins. As revealed by the AFM imaging, the constraint in cell membrane spread on the LC leads to the increase in cell surface roughness and thickness of cell membrane. The biophysical expressions of cells on biocompatible CELC suggested that CELC could be a new class of biological relevant material.