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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Martínez, Raquel Montes
in Cooperation with on an Cooperation-Score of 37%
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Publications (8/8 displayed)
- 20200D polymer nanocomposite carbon-paste electrodes using carbon nanohornscitations
- 2020Customized In Situ Functionalization of Nanodiamonds with Nanoparticles for Composite Carbon-Paste Electrodescitations
- 2018Electrochemical Biosensor Based on Optimized Biocomposite for Organophosphorus and Carbamates Pesticides Detectioncitations
- 2018Carbon nanotube-based nanocomposite sensor tuned with a catechol as novel electrochemical recognition platform of uranyl ion in aqueous samplescitations
- 2017Trends in electrochemical impedance spectroscopy involving nanocomposite transducers: Characterization, architecture surface and bio-sensingcitations
- 2016Highly sensitive electrochemical immunosensor for IgG detection based on optimized rigid biocompositescitations
- 2015Improvement of the detection limit for biosensors: Advances on the optimization of biocomposite compositioncitations
- 2014Towards to the improvement of the analytical response in voltammetric sensors based on rigid compositescitations
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article
Improvement of the detection limit for biosensors: Advances on the optimization of biocomposite composition
Abstract
© 2014 Elsevier B.V. In this work the application of advanced characterization techniques in the development of amperometric biosensors based on biocomposites is described. The optimization of the conductive particle distribution and the amount of the biological material inside the biomaterial have allowed an improvement of the electrochemical properties, regarding the electroanalytical properties such as signal stability and limit of detection. The high signal-to-noise ratio obtained in the electrochemical transduction has allowed enhancing the limit of detection of the biosensor. In the present study, it has been demonstrated the feasibility of electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) for the characterization and optimization of biosensors based on graphite-epoxy-enzyme, using an enzyme model. The optimum biocomposite proportion based on graphite-epoxy which incorporates the enzyme glucose oxidase (GOD) on the matrix ranges between 16% and 17% of graphite using 1% and 2% of enzyme. This range provides the optimal electroanalytical properties. Low limit of detection and good sensitivity have been achieved. Furthermore, confocal laser scanning microscopy was used to visualize the enzyme distribution onto the surface electrode.