| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Mannocci, Francesco
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2021The restoration of root filled teethcitations
- 2019Dental practice in the UK in 2015/2016 - Part 2: Aspects of direct restorations, bleaching, endodontics and paediatric dentistrycitations
- 2018In vitro remineralization of caries-affected dentin after selective carious tissue removalcitations
- 2017Evaluation of dental adhesive systems incorporating an antibacterial monomer eugenyl methacrylate (EgMA) for endodontic restorationscitations
- 2017Bacterial Contamination of Endodontic Materials before and after Clinical Storagecitations
- 2016Influence of a polymerizable eugenol derivative on the antibacterial activity and wettability of a resin composite for intracanal post cementation and core build-up restorationcitations
- 2013Experimental etch-and-rinse adhesives doped with bioactive calcium silicate-based micro-fillers to generate therapeutic resin-dentin interfacescitations
- 2012Contemporary dental practice in the UK in 2008: aspects of direct restorations, endodontics and bleachingcitations
- 2012Synthesis and Preliminary Evaluation of a Polyolefin-based Core for Carrier-based Root Canal Obturationcitations
- 2011Porosity, Micro-Hardness and Morphology of White and Gray Portland Cements in Relation to Their Potential in the Development of New Dental Filling Materialscitations
- 2011Porosity, Micro-Hardness and Morphology of White and Gray Portland Cements in Relation to Their Potential in the Development of New Dental Filling Materialscitations
- 2010Flexural strength of glass fibre-reinforced posts bonded to dual-cure composite resin cementscitations
Places of action
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article
Bacterial Contamination of Endodontic Materials before and after Clinical Storage
Abstract
Introduction The aim of this study was to evaluate the bacterial contamination in endodontic consumables (gutta-percha points, rubber dams, paper mixing pads, caulking agents, and endodontic instrument sponges [EISs]) before and after clinical use and storage. Methods Materials were randomly sampled in triplicates at 3 time points (t0, at package opening; t1, at 7 days; and t2, at 14 days) during their clinical usage. The gutta-percha points and caulking agent (25 mg) were added to 1 mL phosphate-buffered saline (PBS). The rubber dam, paper mixing pad, and EIS were added to 25 mL PBS. After vortexing, centrifuging, and removing the supernatant, the pellet was resuspended in 1 mL PBS, plated on fastidious anaerobic agar, and incubated aerobically and anaerobically. The grown colonies were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The total bacterial load was calculated in the remaining volume (800 μL) from each sample by quantitative polymerase chain reaction after DNA extraction. Results All tested materials showed a varied number of contaminated samples at the 3 time points (except EIS at t0) using MALDI-TOF MS. The most isolated genera were Propionibacterium (42%) and Staphylococcus (32%). By using non–culture-based approaches, all tested materials at the 3 time points (except gutta-percha at t0 and the caulking agent at t0, t1, and t2) carried bacterial DNA. Conclusions The majority of the tested materials harbored bacteria in their samples before and after clinical storage. Nosocomial infection derived from commonly used consumables could have an impact on the outcome of endodontic treatment.