| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Pividori Gurgo, María Isabel
Universitat Autònoma de Barcelona
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (32/32 displayed)
- 2021Development of magnetic nanoparticles modified with new molecularly imprinted polymer (MIPs) for selective analysis of glutathionecitations
- 2020Determination of temporary dye Basic Red 51 in commercial hair dye, river water and wastewater from hairdressing salon using graphite-epoxy composite electrode modified with magnetic nanoparticlescitations
- 2018Electrochemical sensing using magnetic molecularly imprinted polymer particles previously captured by a magneto-sensorcitations
- 2018Electrochemical sensing of methyl parathion on magnetic molecularly imprinted polymercitations
- 2016Use of a composite electrode modified with magnetic particles for electroanalysis of azo dye removed from dyed hair strandscitations
- 2016Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological samplecitations
- 2015DNA polymorphism sensitive impedimetric detection on gold-nanoislands modified electrodescitations
- 2014Electrochemical detection of fluoroquinolone antibiotics in milk using a magneto immunosensorcitations
- 2012Biotin determination in food supplements by an electrochemical magneto biosensorcitations
- 2011Magneto immunoassays for plasmodium falciparum histidine-rich protein 2 related to malaria based on magnetic nanoparticlescitations
- 2011Silver nanocomposite electrode modified with hexacyanoferrate. Preparation, characterization and electrochemical behaviour towards substituted anilinescitations
- 2010Preparation and Characterization of Graphite-Epoxy Composite Modified with Zinc Hexacyanoferrate and Their Electrochemical Behaviour in Presence of Substituted Anilinescitations
- 2009Electrochemical immunosensor for the diagnosis of celiac diseasecitations
- 2009Double-tagging polymerase chain reaction with a thiolated primer and electrochemical genosensing based on gold nanocomposite sensor for food safetycitations
- 2009Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensorscitations
- 2007Electrochemical magneto immunosensing of antibiotic residues in milkcitations
- 2007Bioaffinity platforms based on carbon-polymer biocomposites for electrochemical biosensingcitations
- 2007Electrochemical biosensing of pesticide residues based on affinity biocomposite platformscitations
- 2007Application of the avidin-biotin interaction to immobilize DNA in the development of electrochemical impedance genosensorscitations
- 2007In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogenscitations
- 2006Impedimetric genosensors for the detection of DNA hybridizationcitations
- 2006Genomagnetic assay based on label-free electrochemical detection using magneto-composite electrodescitations
- 2006Electrochemical magnetoimmunosensing strategy for the detection of pesticides residuescitations
- 2006Extractant assisted synthesis of polymer stabilized platinum and palladium metal nanoparticles for sensor applicationscitations
- 2006Electrochemical biosensing based on universal affinity biocomposite platformscitations
- 2005Magnetically trigged direct electrochemical detection of DNA hybridization using Au67 quantum dot as electrical tracercitations
- 2005Electrochemical genosensing based on rigid carbon composites. A reviewcitations
- 2004Renewable Protein A modified graphite-epoxy composite for electrochemical immunosensingcitations
- 2004Rigid carbon composites: A new transducing material for label-free electrochemical genosensingcitations
- 2003Graphite-epoxy platforms for electrochemical genosensingcitations
- 2003Rapid electrochemical genosensor assay using a streptavidin carbon-polymer biocomposite electrodecitations
- 2003Graphite-epoxy composites as a new transducing material for electrochemical genosensingcitations
Places of action
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article
Renewable Protein A modified graphite-epoxy composite for electrochemical immunosensing
Abstract
A novel rigid and renewable transducing material for electrochemical immunosensing, based on Protein A bulk-modified graphite-epoxy biocomposite (ProtA-GEB) is reported. Protein A is able to bind to the Fc region of antibodies and provide an affinity matrix for antibody immobilisation onto the transducer. The rigid conducting biocomposite acts not only as a transducer, but also as a reservoir for protein A. After use, the electrode surface can be renewed by a simple polishing procedure, highlighting a clear advantage of this new approach with respect to classical immunoassays. The performance of ProtA-GEB transducers was compared with surface-modified transducers based on a simple dry adsorption procedure, where both Protein A and directly the antibody were adsorbed onto the surface of graphite-epoxy composite (ProtA/GEC and IgG/GEC, respectively). The application of the new biocomposite material in electrochemical immunosensing was studied using a model competitive immunoassay. The immunological reaction was detected using an enzymatic-labeling procedure together with the amperometric detection through a suitable substrate (H 2O2) for the enzyme (HRP). The enzymatic labelling was performed using a two-step procedure based on the biotin/streptavidin interaction as well as a one-step procedure using an antibody labelled with the enzyme. Electrochemical and microscopic characterisation of ProtA-GEB transducer, optimisation of the immunosensor design as well as the stability of this material are also reported. © 2004 Elsevier B.V. All rights reserved.