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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Granja, Pl
in Cooperation with on an Cooperation-Score of 37%
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Publications (6/6 displayed)
- 2015Understanding the composition-structure-bioactivity relationships in diopside (CaO center dot MgO center dot 2SiO(2))-tricalcium phosphate (3CaO center dot P2O5) glass systemcitations
- 2012Biocompatibility and biodegradation of polycaprolactone-sebacic acid blended gelscitations
- 2010Anti-adhesion and antiproliferative cellulose triacetate membrane for prevention of biomaterial-centred infections associated with Staphylococcus epidermidiscitations
- 2009Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectorscitations
- 2006Functionalization of chitosan membranes through phosphorylation: Atomic force microscopy, wettability, and cytotoxicity studiescitations
- 2001Staphylococcus epidermidis RP62A adhesion to chemically modified cellulose derivativescitations
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article
Osteogenic differentiation of mesenchymal stem cells using PAMAM dendrimers as gene delivery vectors
Abstract
This paper reports the use of different generations of polyamidoamine (PAMAM) dendrimers for the in vitro transfection of mesenchymal stem cells (MSCs). A systematic study was carried out on the transfection efficiency achieved by the PAMAM dendrimers using a p-galactosidase reporter gene system. Transfection results were shown to be dependent upon the generation of dendrimers, the amine to phosphate group ratio and the cell passage number. In all cases, the transfection efficiency was very low. Nevertheless, it was hypothesized that a low transfection level could be sufficient to promote the in vitro differentiation of MSCs towards the osteoblastic lineage. To address this possibility, dendrimers carrying the human bone morphogenetic protein-2 (hBMP-2) gene-containing plasmid were used. All quantitative (alkaline phosphatase activity, osteocalcin secretion and calcium deposition) and qualitative (von Kossa staining) osteogenic markers were significantly stronger in transfected cells when compared to non-transfected ones. This study not only clearly demonstrates that a low transfection level can be sufficient for inducing in vitro differentiation of MSCs to the osteoblast phenotype but also highlights the importance of focusing research on the development of gene delivery vectors in the concrete application.