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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Bruno, A.
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Topics
Publications (10/10 displayed)
- 2017Oxadiazole-carbazole polymer (POC)-Ir(ppy)3 tunable emitting compositescitations
- 2016Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution.citations
- 2015Physicochemical evolution of nascent soot particles in a laminar premixed flame: From nucleation to early growthcitations
- 2015PbS nanocrystals in hybrid systems for solar cell applicationscitations
- 2015Further details on particle inception and growth in premixed flamescitations
- 2014Spectroscopic evaluation of mixing and crystallinity of fullerenes in bulk heterojunctionscitations
- 2013Preparation and characterization of novel nanocomposites of WS2 nanotubes and polyfluorene conductive polymercitations
- 2013Emission properties of polydioctylfluorene and InP/ZnS quantum dots nanocomposites devices
- 2013Microscopic and spectroscopic investigation of MoS2 nanotubes/P3HT nanocompositescitations
- 2013White light-emitting nanocomposites based on an oxadiazole-carbazole copolymer (POC) and InP/ZnS quantum dotscitations
Places of action
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article
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution.
Abstract
The flagellated protozoan Dientamoeba fragilis is often detected in humans with gastrointestinal symptoms, but it is also commonly found in healthy subjects. As for other intestinal protozoa, the hypothesis that genetically dissimilar parasite isolates differ in their ability to cause symptoms has also been raised for D. fragilis. To date, only two D. fragilis genotypes (1 and 2) have been described, of which genotype 1 largely predominates worldwide. However, very few markers are available for genotyping studies and therefore the extent of genetic variation among isolates remains largely unknown. Here, we performed metagenomics experiments on two D. fragilis-positive stool samples, and identified a number of candidate markers based on sequence similarity to the phylogenetically related species Trichomonas vaginalis. Markers corresponding to structural genes and to genes encoding for proteases were selected for this study, and PCR experiments confirmed their belonging to the D. fragilis genome; two previously described markers (small subunit ribosomal DNA and large subunit of RNA polymerase II) were also included. Using this panel of markers, 111 isolates of human origin were genotyped, all of which, except one, belonged to genotype 1. These isolates had been collected at different times from symptomatic and asymptomatic persons of different age groups in Italy, Denmark, Brazil and Australia. By sequencing approximately 160kb from 500 PCR products, a very low level of polymorphism was observed across all the investigated loci, suggesting the existence of a major clone of D. fragilis with a widespread geographical distribution.