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Cardoso, Sm
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article
Characterization of galactooligosaccharides produced by beta-galactosidase immobilized onto magnetized Dacron
Abstract
Bioconversions using enzymes immobilized in magnetic supports present significant advantages due to the easy separation of the enzyme from the reaction mixture and the simplicity and low cost of the support preparation. The characterization of the oligosaccharide mixture obtained by the action of beta-galactosidase covalently attached, via glutaraldehyde, to a hydrazide-Dacron-magnetite composite is presented. The fractionation of the oligosaccharide mixture by high performance liquid chromatography, followed by the analysis of the purified compounds by mass spectrometry and nuclear magnetic resonance spectroscopy permitted the identification of glucose, galactose, lactose and a hexose disaccharide containing a 1 -> 6 linkage. Also, the following GOS were identified: beta-D-Galp-(1 -> 6)-beta-D-Galp-(1 -> 4)-Glcp, beta-D-Galp-(1 -> 4)-[beta-D-Galp-(1 -> 6)]-Glcp and beta-D-Galp-(1 -> 6)-beta-D-Galp-(1 -> 6)-beta-D-Galp-(1 -> 4)-Glcp. When GOS yield (26.2%) and kinetics of biotransformation of lactose by the Dacron immobilized beta-galactosidase were compared with values obtained for the enzyme immobilized in other magnetic supports, similar behaviour was observed.