| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Pintado, Maria Manuela
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2024ATR-FTIR as a high throughput tool for quick screening of lipolysis in 3T3- L1 adipocytes
- 2024Unravelling the effects of extrusion and drying temperatures on the radical scavenging capacity of aquafeeds supplemented with mango and pineapple by-productscitations
- 2023Sustainable & integrative approach for valorisation of citrus by-products in the Mediterranean
- 2021Chitosan-olive oil microparticles for phenylethyl isothiocyanate delivery
- 2021Chitosan-olive oil microparticles for phenylethyl isothiocyanate deliverycitations
- 2021Valorisation of mussel mytilus galloprovincialis meat waste to produce bioactive extracts by enzymatic hydrolysis
- 2019Collagen-based bioactive hydrolysates production from blue shark skin
- 2019Organic nanocomposites for the delivery of bioactive moleculescitations
- 2019Organic nanocomposites for the delivery of bioactive moleculescitations
- 2018Combination of PLGA nanoparticles with mucoadhesive guar-gum films for buccal delivery of antihypertensive peptidecitations
- 2015Evaluation of the interactions between rosmarinic acid and bovine milk caseincitations
- 2015Study of the interactions between rosmarinic acid and bovine milk whey protein α-Lactalbumin, β-Lactoglobulin and Lactoferrincitations
- 2013A novel direct contact method for the assessment of the antimicrobial activity of dental cementscitations
Places of action
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article
Study of the interactions between rosmarinic acid and bovine milk whey protein α-Lactalbumin, β-Lactoglobulin and Lactoferrin
Abstract
Incorporation of polyphenols in dairy food is being considered as way to develop oral nutraceutical formulation due to their antioxidant and anti-inflammatory activities. However, polyphenols can interact with proteins, which gives rise to a significant loss of their biological properties. The objective of this research was to study the interactions of rosmarinic acid (RA) and bovine milk whey protein (i.e. α-Lactalbumin, β-Lactoglobulin and Lactoferrin). Radical quenching assay (ABTS), optical density, liquid chromatography (HPLC and FPLC), dynamic light scattering (DLS) and zeta-potential, Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC) were used for the screening of the interactions at 0, 3, and 24 h of storage at 4. °C. Interactions were assessed both at neutral pH of the complexes in water and acidic pH. 3 and 4.5. Results show the occurrence of non-covalent interactions between RA and whey proteins, such as hydrophobic, hydrogen bonding, and dipole-dipole type. Radical quenching activity of RA decreased in the presence of milk proteins yet at the initial time especially in the case of α-Lactalbumin, meaning that the amount of free polyphenol diminished. Complex dimension was different depending on pH, and on primary and secondary structures of proteins. Interactions showed to be favored at the lowest pH, and reversible in all cases; nonetheless, complex RA-proteins were more stable than proteins alone.