| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Rajala, Pauliina
VTT Technical Research Centre of Finland
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (27/27 displayed)
- 2024Integrating double-labeling HCR-FISH into a multidisciplinary pipeline for biofouling assessment on austenitic stainless steel in brackish seawater circuitcitations
- 2023Applied DNA HCR-FISH for Biofilm Distribution Imaging on Stainless Steel in Brackish Seawater
- 2022Sulfate-dependant microbially induced corrosion of mild steel in the deep sea:a 10-year microbiome studycitations
- 2022Fluctuation in deep groundwater chemistry and microbial community and their impact on corrosion of stainless-steelscitations
- 2021Corrosion of copper in sulphide containing environment: the role and properties of sulphide films – Annual report 2020
- 2019Corrosion and biofouling tendency of carbon steel in anoxic groundwater containing sulphate reducing bacteria and methanogenic archaeacitations
- 2018Copper corrosion monitoring by electrical resistance probes in anoxic groundwater environment in the presence and absence of sulfate reducing bacteriacitations
- 2018Kinetic properties of the passive film on copper in the presence of sulfate-reducing bacteriacitations
- 2018Ennoblement, corrosion, and biofouling in brackish seawater:Comparison between six stainless steel gradescitations
- 2018Ennoblement, corrosion, and biofouling in brackish seawatercitations
- 2018Real-time corrosion monitoring system under in situ conditions of crystalline groundwater
- 2018Corrosion of copper in anoxic ground water in the presence of SRB
- 2017Microbially induced corrosion (MIC) of carbon steel and stainless steels grades EN 1.4301 and EN 1.4432 in deep bedrock environment
- 2017The effect of hypochlorite treatment on stainless steel performance and fouling in cooling water cycles
- 2017EIS study on aerobic corrosion of copper in ground water: influence of micro-organismscitations
- 2017Microbial fouling and corrosion of carbon steel in deep anoxic alkaline groundwatercitations
- 2017Microbially-induced corrosion of carbon steel in a geological repository environment
- 2017Corrosion of stainless steels AISI 304 and AISI 316 induced by sulfate reducing bacteria in anoxic groundwater
- 2017Corrosion Behavior of Copper in Simulated Anoxic Groundwater Inoculated with Sulfate Reducing Bacteria and Methanogens
- 2016Corrosion and biofouling on stainless steels in Baltic sea water environment:a cooling water pilot study
- 2016Influence of Chlorination and Choice of Materials on Fouling in Cooling Water System under Brackish Seawater Conditionscitations
- 2016Biofouling on Coated Carbon Steel in Cooling Water Cycles Using Brackish Seawatercitations
- 2016Corrosion and biofouling on stainless steels in Baltic sea water environment
- 2015Real-Time Electrochemical Measurements of Carbon Steel in Ground Water with Sulfate Reducing Bacteria Enrichment
- 2014Microbial diversity and corrosion behaviour of carbon steel and stainless steel after one-year exposure in alkaline ground water
- 2014Microbially induced corrosion of carbon steel and stainless steel in alkaline ground water -composition and metabolic functionality of biofilm
- 2014Corrosion of copper in anaerobic groundwater in the presence of SRB
Places of action
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article
Integrating double-labeling HCR-FISH into a multidisciplinary pipeline for biofouling assessment on austenitic stainless steel in brackish seawater circuit
Abstract
This study modified and integrated a bioimaging method of hybridization chain reaction fluorescence in situ hybridization (HCR-FISH) into a pipeline for assessing biofouling on stainless steel (SS). A modified protocol of double-labeling HCR-FISH was directly applied to two surface types of SS grade EN 1.4404 to detect localized bacteria and sulfate-reducing bacteria (SRB) by targeting bacterial 16 S rRNA genes and dissimilatory sulfite reductase (dsrB) genes, respectively. The protocol was first validated using microbial pure cultures and materials before being integrated into a biofouling assessment pipeline of SS in a laboratory-scale brackish water circuit, incorporating electrochemical, surface, and molecular biology characterization analyses. The double-labeling HCR-FISH improved bioimaging of surface biofilm morphology and microbial distribution, surpassing monochrome staining methods. This method was compatible and complemented other microscopy techniques and molecular biological analyses, providing additional insights into the biofilms and deposits on the alloy surfaces. The implemented assessment pipeline for biofouling determination frequently detected the ennoblement phenomenon in the evolution of marine biofilm on SS surfaces. However, within the experimental timeframe, microbial activities in the brackish seawater circuit did not flourish significantly, resulting in minimal impact on the steel material. Additionally, surface type and roughness may correlate with microbial adhesion, biofilm growth, and the deformation of passivation layers in SS. Despite abundant sessile bacteria, particularly opportunistic microorganisms, on the steel surfaces, no direct correlations with biodeterioration phenomena or influences of surface roughness of an alloy and the presence of biofilm were conclusively established.