| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Ratia-Hanby, Vilma L.
VTT Technical Research Centre of Finland
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2024Integrating double-labeling HCR-FISH into a multidisciplinary pipeline for biofouling assessment on austenitic stainless steel in brackish seawater circuitcitations
- 2024The evolution of subsurface deformation and tribological degradation of a multiphase Fe-based hardfacing induced by sliding contact
- 2024Backgrounds for Studying Impact of Different Water Environments on Welded Steels for Low and Intermediate-Level Waste Repositories in Finland
- 2024The effect of alloying and surface roughness on biofouling of stainless steels in Baltic Sea brackish seawater
- 2023Penetration of corrosive species into copper exposed to simulated O2-free groundwater by time-of-flight secondary ion mass spectrometry (ToF-SIMS)citations
- 2023Characterization of surface films that develop on pre-oxidized copper in anoxic simulated groundwater with sulphidecitations
- 2023Applied DNA HCR-FISH for Biofilm Distribution Imaging on Stainless Steel in Brackish Seawater
- 2021Microstructural characterisation of subsurface deformation and the degradation of Stellite 6 induced by self-mated sliding contact in a simulated PWR environmentcitations
- 2021Corrosion of copper in sulphide containing environment: the role and properties of sulphide films – Annual report 2020
- 2021Corrosion-induced microstructure degradation of copper in sulfide-containing simulated anoxic groundwater studied by synchrotron high-energy X-ray diffraction and ab-initio density functional theory calculationcitations
- 2019Research methods for the evaluation of the relevance of application oriented laboratory wear tests
- 2015Behavior of martensitic wear resistant steels in abrasion and impact wear testing conditions
- 2015The effect of impact conditions on the wear and deformation behavior of wear resistant steelscitations
Places of action
| Organizations | Location | People |
|---|
article
Integrating double-labeling HCR-FISH into a multidisciplinary pipeline for biofouling assessment on austenitic stainless steel in brackish seawater circuit
Abstract
This study modified and integrated a bioimaging method of hybridization chain reaction fluorescence in situ hybridization (HCR-FISH) into a pipeline for assessing biofouling on stainless steel (SS). A modified protocol of double-labeling HCR-FISH was directly applied to two surface types of SS grade EN 1.4404 to detect localized bacteria and sulfate-reducing bacteria (SRB) by targeting bacterial 16 S rRNA genes and dissimilatory sulfite reductase (dsrB) genes, respectively. The protocol was first validated using microbial pure cultures and materials before being integrated into a biofouling assessment pipeline of SS in a laboratory-scale brackish water circuit, incorporating electrochemical, surface, and molecular biology characterization analyses. The double-labeling HCR-FISH improved bioimaging of surface biofilm morphology and microbial distribution, surpassing monochrome staining methods. This method was compatible and complemented other microscopy techniques and molecular biological analyses, providing additional insights into the biofilms and deposits on the alloy surfaces. The implemented assessment pipeline for biofouling determination frequently detected the ennoblement phenomenon in the evolution of marine biofilm on SS surfaces. However, within the experimental timeframe, microbial activities in the brackish seawater circuit did not flourish significantly, resulting in minimal impact on the steel material. Additionally, surface type and roughness may correlate with microbial adhesion, biofilm growth, and the deformation of passivation layers in SS. Despite abundant sessile bacteria, particularly opportunistic microorganisms, on the steel surfaces, no direct correlations with biodeterioration phenomena or influences of surface roughness of an alloy and the presence of biofilm were conclusively established.