• Mauffret, Aourell
• Thomas, Federle
• Andre, Rottiers
• Miriam, Hampel
• Gillan David, C.
• Ali, Temara

Abstract

An innovative biodegradation test system was developed in order to fill the current gap for cost effective and environmentally relevant tools to assess marine biodegradability. Glass beads were colonized by a biofilm in an open flow-through system of seawater with continuous pre-exposure to Linear Alkylbenzene Sulfonate (LAS) (20 mu g/L). Thereafter, such colonized beads were added as inoculum in different test systems. [(14)C]-LAS (5-100 mu g/L) was added and primary and ultimate biodegradation were assessed. The bacterial density collected on the beads (10(9) bact./mL beads) was ca. 3 orders of magnitude higher than the typical seawater content. The LAS mineralization lag phase duration decreased from 55 to <1 days and the mineralization extent increased from 53 to 90% as the colonized beads volume increased from 10 to 275 mL This is the first demonstration of marine bacteria's ability to mineralize LAS. On the opposite, less than 13% LAS was mineralized in seawater only. The colonized beads possibly enhanced the probability to encounter the full degraders' consortium in a low volume of seawater (100 ml.).

Topics

• density
• impedance spectroscopy
• phase
• glass
• glass
• laser emission spectroscopy
• liquid-assisted grinding
• laser absorption spectroscopy