• Camilleri, Josette
• Meerbeek, Bart Van
• Li, Xin
• Meschi, Nastaran
• Lambrechts, Paul
• Gorp, Gertrude Van

Abstract

Objective: The aim of this study was to evaluate the bioactivity potential of an hydraulic calcium-silicate cement, Pure Portland Cement Med-PZ (Medcem, Weinfelden, Switzerland: ‘MPC’), applied in a tooth extracted because of failed regenerative endodontic procedures (REP) and by means of ex vivo (EV) specimens.<br/><br/>Methods: Ten EV cylindrical dentin cavities were prepared and filled with MPC and stored for 1 month in distilled water (DW), Hank’s balanced salt solution (HBSS), Dulbecco’s phosphate-buffered saline (DPBS), simulated body fluid (SBF), versus no media (NM) serving as control. Six additional EV specimens were filled with MPC and exposed for 2 weeks to leucocyte-and-platelet-rich fibrin (LPRF)-clot (C), LPRF-membrane (M) and LPRF-exudate (E). MPC in the EV specimens and in the coronal part of the REP tooth was analyzed by means of micro-Raman spectroscopy (MR), scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS).<br/><br/>Results: SEM showed rough crystallite surfaces for the EV samples and a porous surface for the REP tooth. EDS of the EV samples revealed prominent peaks for Ca, Si and O. Storage in HBSS, DPBS, SBF, exposure to LPRF and the REP tooth showed considerable amounts of P as well. MR exhibited vibrations of phosphate (DPBS, SBF), carbonated hydroxyapatite (DPBS, SBF), calcium carbonate (DW, HBSS, NM, REP-tooth, LPRF-E), oxidized (ferric) proteins (LPRF-E/C/M) and the amide III band (all samples). Hence, only storage of MPC in DPBS and SBF for 1 month revealed bioactivity.<br/><br/>Significance: The environmental conditions, namely the laboratory and clinical settings, affect the bioactivity potential of MPC.<br/>

Topics

• porous
• surface
• scanning electron microscopy
• cement
• Energy-dispersive X-ray spectroscopy
• Calcium
• Raman spectroscopy
• bioactivity