• Tezvergil-Mutluay, Arzu
• Tjäderhane, Leo
• Sinhoreti, Mário Alexandre Coelho
• Stape, Thiago Henrique Scarabello
• Martins, Luís Roberto Marcondes
• Abuna, Gabriel

Abstract

<p>Objective. To determine whether bonding effectiveness and hybrid layer integrity on acid-etched dehydrated dentin would be comparable to the conventional wet-bonding technique through new dentin biomodification approaches using dimethyl sulfoxide (DMSO).</p><p>Methods. Etched dentin surfaces from extracted sound molars were randomly bonded in wet or dry conditions (30 s air drying) with DMSO/ethanol or DMSO/H2O as pretreatments using a simplified (Scotchbond Universal Adhesive, 3M ESPE: SU) and a multi-step (Adper Scotchbond Multi-Purpose, 3M ESPE: SBMP) etch-and-rinse adhesives. Untreated dentin surfaces served as control. Bonded teeth (n=8) were stored in distilled water for 24 h and sectioned into resin-dentin beams (0.8 mm(2)) for microtensile bond strength test and quantitative interfacial nanoleakage analysis (n = 8) under SEM. Additional teeth (n = 2) were prepared for micropermeability assessment by CFLSM under simulated pulp ar pressure (20 cm H2O) using 5 mM fluorescein as a tracer. Microtensile data was analyzed by 3-way ANOVA followed by Tukey Test and nanoleakage by Kruskal-Wallis and Dunn-Bonferroni multiple comparison test (alpha = 0.05).</p><p>Results. While dry-bonding of SBMP produced significantly lower bond strengths than wet-bonding (p</p><p>Conclusion. DMSO pretreatments may be used as a new suitable strategy to improve bonding of water-based adhesives to demineralized air-dried dentin beyond conventional wetbonding. Less porous resin-dentin interfaces with higher bond strengths on air-dried etched dentin were achieved; nonetheless, overall efficiency varied according to DMSO's co-solvent and adhesive type.</p><p>Clinical significance. DMSO pretreatments permit etched dentin to be air-dried before hybridization facilitating residual water removal and thus improving bonding effectiveness. This challenges the current paradigm of wet-bonding requirement for the etch-and-rinse approach creating new possibilities to enhance the clinical longevity of resin-dentin interfaces. (C) 2018 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.</p>

Topics

• porous
• surface
• scanning electron microscopy
• laser emission spectroscopy
• strength
• resin
• drying