• Paiva, Alexandre
• Silva, Joana M.
• Duarte, Ana Rita C.
• Castro, Vânia I. B.
• Craveiro, Rita
• Reis, Rui L.

Abstract

Natural deep eutectic systems (NADES) are mostly composed of natural primary metabolites such as sugars, sugar alcohols, organic acids, amino acids and amines. These simple molecules have been identified in animals living in environments with extreme temperature amplitudes, being responsible for their survival at negative temperatures during winter. Herein, we report for the first time the use of NADES based on trehalose (Treh) and glycerol (Gly) in cryopreservation, as cryoprotective agents (CPA). The evaluation of the thermal behaviour of these eutectic systems, showed that NADES have a strong effect on the water crystallization/freezing and melting process, being able to reduce the number of ice crystals and hence ice crystal damage in cells, which is a crucial parameter for their survival, upon freezing. Using this NADES as CPA, it is possible to achieve similar or even better cellular performance when compared with the gold standard for cryopreservation dimethyl sulfoxide (DMSO). In this sense, this work relates the physical properties of the NADES with their biological performance in cryopreservation. Our comprehensive strategy results in the demonstration of NADES as a promising nontoxic green alternative to the conventional CPA's used in cryopreservation methods. ; The research leading to these results has received funding provided by Portuguese Foundation for Science and Technology (FCT), through project PTDC/BBB-EBB/1676/2014 – DesZyme. Funding from the European Union Horizon 2020 program has also been, granted through the project Des.solve (ERC consolidator), ERC-2016-COG 725034. J.M.S. would also like to acknowledge the financial support by the FCT through the post-doctoral grant with reference number SFRH/BPD/ 116779/2016. ; info:eu-repo/semantics/publishedVersion

Topics

• impedance spectroscopy
• gold
• amine
• crystallization
• alcohol
• constant potential amperometry