| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Fonseca, Fernanda
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 2024Chickpeas’ Cooking Wastewater as an Alternative Source of Galacto-Oligosaccharides for Improving the Freeze-Dried Resistance of Lactic Acid Bacteriacitations
- 2023Differential scanning calorimetry applied to the preservation of bacteria and mammalian cells
- 2023Insights into the mechanisms of L. salivarius CECT5713 resistance to freeze-dried storagecitations
- 2020Bacterial cell adaptation to environmental stress during fermentation improves cryo-and drying resistance: A focus on membrane properties
- 2019Physical events during cryopreservation: consequences on cells’ post-thaw performance and on cryobiological protocols optimisation
- 2015Initial measurement of the intracellular glass transition of a mammalian cell type under cryopreservation conditions
- 2011Quality degradation of lactic acid bacteria during the freeze drying process : experimental study and mathematical modelling
- 2005Effect of sucrose and maltodextrin on the physical properties and survival of air-dried Lactobacillus bulgaricus : an in situ fourier transform infrared spectroscopy studycitations
- 2004Collapse temperature of freeze-dried Lactobacillus bulgaricus suspensions and protective mediacitations
Places of action
| Organizations | Location | People |
|---|
document
Initial measurement of the intracellular glass transition of a mammalian cell type under cryopreservation conditions
Abstract
A key physical event during the cooling of cells is the temperature at which the intracellular compartment undergoes vitrification. Recently (http://dx. doi.org/10.1371/journal.pone.0066207) we have measured the intracellular glass transition temperature (Tg) of bacteria using differential scanning calorimetry (DSC). Here we report the first data on the intracellular Tg of a mammalian cell cooled with and without a cryoprotectant (Me2SO). Jurkat cells (an immortalized T cell line) were concentrated and a pellet of approximately 30 mg cooled in a DSC, SnowMax was added to induce ice nucleation. On warming an intracellular glass transition was detected by the deviation in the first derivative of the heat flow, associated with a change in heat capacity as the cells devitrify at Tg. The transition temperature measured by DSC is Tg', that is the Tg of the maximally freezeconcentrated intracellular matrix. In the presence of Me2SO intracellular Tg was measured to be - 40 C, in controls (no Me2SO) Tg occurred at higher temperatures. In further experiments the temperature of the lipid phase transitions were analyzed by FTIR, and were measured to occur above freezing temperatures. The implications of the measured values of Tg’ for the basic cryobiology of mammalian cells will be discussed.