| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Nogués, C.
Universitat Autònoma de Barcelona
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (17/17 displayed)
- 2023Accelerated biodegradation of FeMn porous alloy coated with ZnOcitations
- 2023Surface Modified β-Ti-18Mo-6Nb-5Ta (wt%) Alloy for Bone Implant Applications:citations
- 2023Hierarchical Surface Pattern on Ni‐Free Ti‐Based Bulk Metallic Glass to Control Cell Interactionscitations
- 2022Biodegradable porous FeMn(-xAg) alloys:citations
- 2018Cytocompatibility assessment of Ti-Zr-Pd-Si-(Nb) alloys with low Young's modulus, increased hardness, and enhanced osteoblast differentiation for biomedical applications
- 2017Study of Galfenol direct cytotoxicity and remote microactuation in cellscitations
- 2017Mechanical properties, corrosion performance and cell viability studies on newly developed porous Fe-Mn-Si-Pd alloyscitations
- 2016Effect of surface modifications of Ti40Zr10Cu38Pd12 bulk metallic glass and Ti-6Al-4V alloy on human osteoblasts in vitro biocompatibilitycitations
- 2016Novel Fe-Mn-Si-Pd alloys: Insights into mechanical, magnetic, corrosion resistance and biocompatibility performancescitations
- 2015Nanostructured Ti-Zr-Pd-Si-(Nb) bulk metallic composites: Novel biocompatible materials with superior mechanical strength and elastic recoverycitations
- 2015Controlling colloidal stability of silica nanoparticles during bioconjugation reactions with proteins and improving their longer-term stability, handling and storagecitations
- 2014In vitro biocompatibility assessment of Ti40Cu38Zr10Pd12 bulk metallic glasscitations
- 2014Optimized immobilization of lectins using self-assembled monolayers on polysilicon encoded materials for cell taggingcitations
- 2013On the biodegradability, mechanical behavior, and cytocompatibility of amorphous Mg72Zn23Ca5 and crystalline Mg70Zn23Ca5Pd2 alloys as temporary implant materialscitations
- 2013Novel Ti-Zr-Hf-Fe nanostructured alloy for biomedical applicationscitations
- 2012Efficient biofunctionalization of polysilicon barcodes for adhesion to the zona pellucida of mouse embryoscitations
- 2012Improved mechanical performance and delayed corrosion phenomena in biodegradable Mg-Zn-Ca alloys through Pd-alloyingcitations
Places of action
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article
Optimized immobilization of lectins using self-assembled monolayers on polysilicon encoded materials for cell tagging
Abstract
Self-assembled monolayers (SAMs) have been used for the preparation of functional microtools consisting of encoded polysilicon barcodes biofunctionalized with proteins of the lectin family. These hybrid microtools exploit the lectins ability for recognizing specific carbohydrates of the cell membrane to give an efficient system for cell tagging. This work describes how the control of the methodology for SAM formation on polysilicon surfaces followed by lectin immobilization has a crucial influence on the microtool biofunction. Several parameters (silanization time, silane molar concentration, type of solvent or deposition methodology) have been studied to establish optimal function. Furthermore, silanes incorporating different terminal groups, such as aldehyde, activated ester or epoxide groups were tested in order to analyze their chemical coupling with the biomolecules, as well as their influence on the biofunctionality of the immobilized protein. Two different lectins - wheat germ agglutinin (WGA) and phytohemagglutinin (PHA-L) - were immobilized, because they have different and specific cell recognition behaviour and exhibit different cell toxicity. In this way we can assess the effect of intrinsic bulk toxicity with that of the cell compatibility once immobilized as well as the importance of cell affinity. A variety of nanometrical techniques were used to characterize the active surfaces, and lectin immobilization was quantified using ultraviolet-visible absorption spectroscopy (UV-vis) and optical waveguide light mode spectroscopy (OWLS). Once the best protocol was found, WGA and PHA were immobilized on polysilicon coded barcodes, and these microtools showed excellent cell tagging on living mouse embryos when WGA was used. © 2013 Elsevier B.V.