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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Desmet, Gert
Vrije Universiteit Brussel
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2024Detailed analysis of the effective and intra-particle diffusion coefficient of proteins at elevated pressure in columns packed with wide-pore core-shell particlescitations
- 2023Development of a 3D-Printable, Porous, and Chemically Active Material Filled with Silica Particles and its Application to the Fabrication of a Microextraction Devicecitations
- 2022Measurement of the molecular diffusion coefficient and the effective longitudinal diffusion under supercritical fluid chromatography conditions in packed bed columnscitations
- 2022Review of recent insights in the measurement and modelling of the B-term dispersion and related mass transfer properties in liquid chromatographycitations
- 2022Vacuum-driven assembly of electrostatically levitated microspheres on perforated surfacescitations
- 2021Measurement and modelling of the intra-particle diffusion and b-term in reversed-phase liquid chromatographycitations
- 2020Spatial Segregation of Microspheres by Rubbing-Induced Triboelectrification on Patterned Surfacescitations
- 2020A Methodology for the Estimation and Modelling of the Obstruction Factor in the Expression for Mesopore Diffusion in Reversed-Phase Liquid Chromatography Particlescitations
- 2019Study of peak capacities generated by a porous layered radially elongated pillar array column coupled to a nano-LC systemcitations
- 2011High-resolution separations of protein isoforms with liquid chromatography time-of-flight mass spectrometry using polymer monolithic capillary columns
- 2011Ultra-high-resolution separations of intact proteins with LC-TOF-MS using polymer monolithic columns
- 2008Errors involved in the Existing B-term Expressions for the Longitudinal Diffusion in Fully Porous Chromatographic Media. Part II: Experimental data in Packed Columns and Surface Diffusion Measurements
Places of action
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article
Measurement and modelling of the intra-particle diffusion and b-term in reversed-phase liquid chromatography
Abstract
In an ongoing effort to better understand the underlying mechanisms of band broadening in particle-packed reversed-phase liquid chromatography columns, new models for intra-particle diffusion, representing an adsorption- and partition-type retention behavior, are proposed. These models assume the mesoporous zone inside the particles is subdivided in four distinct regions: a fraction f(1) filled with bulk mobile phase, a fraction f(2) enriched in pure organic modifier extending outside the stationary phase layer, a fraction f(3) comprising the liquid surrounding the alkyl chains and a fraction f(4) consisting of the stationary phase alkyl chains. Intra-particle diffusion is calculated as a residence time weighted average of the diffusion in these different regions.Experimental procedures and models are proposed to determine the volumes of these four regions and applied to three reversed-phase liquid chromatography columns with different pore sizes (80 angstrom versus 300 angstrom) and different stationary phase types (C-18 versus C-8). The newly proposed models are then applied to predict the intra-particle diffusion of butyrophenone across a wide range of retention factors (1 <= k" <= 40) in each of these columns. These predictions are compared to experimental data that are extracted from the effective diffusion coefficients of butyrophenone obtained via peak parking experiments.It is demonstrated that both adsorption- and partition-type models for intra-particle diffusion model the actual behavior of the test compound well, and require the determination of only one (partition) or two (adsorption) fitting factors: the obstruction to free movement the analytes experience from the alkyl chains in the retained state (partition and adsorption) and in the unretained state (adsorption). Finally, it is demonstrated that the major contributor to the intra-particle diffusion of retained compounds (k" > 2) is the diffusion these analytes undergo when retained in the organic-modifier enriched zone surrounding the alkyl chains (partition model) or when adsorbed onto the alkyl chains (adsorption model), confirming that surface diffusion plays an important role in the mass transfer of retained compounds in reversed-phase liquid chromatography columns. (C) 2020 Elsevier B.V. All rights reserved.