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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Desmet, Gert
Vrije Universiteit Brussel
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2024Detailed analysis of the effective and intra-particle diffusion coefficient of proteins at elevated pressure in columns packed with wide-pore core-shell particlescitations
- 2023Development of a 3D-Printable, Porous, and Chemically Active Material Filled with Silica Particles and its Application to the Fabrication of a Microextraction Devicecitations
- 2022Measurement of the molecular diffusion coefficient and the effective longitudinal diffusion under supercritical fluid chromatography conditions in packed bed columnscitations
- 2022Review of recent insights in the measurement and modelling of the B-term dispersion and related mass transfer properties in liquid chromatographycitations
- 2022Vacuum-driven assembly of electrostatically levitated microspheres on perforated surfacescitations
- 2021Measurement and modelling of the intra-particle diffusion and b-term in reversed-phase liquid chromatographycitations
- 2020Spatial Segregation of Microspheres by Rubbing-Induced Triboelectrification on Patterned Surfacescitations
- 2020A Methodology for the Estimation and Modelling of the Obstruction Factor in the Expression for Mesopore Diffusion in Reversed-Phase Liquid Chromatography Particlescitations
- 2019Study of peak capacities generated by a porous layered radially elongated pillar array column coupled to a nano-LC systemcitations
- 2011High-resolution separations of protein isoforms with liquid chromatography time-of-flight mass spectrometry using polymer monolithic capillary columns
- 2011Ultra-high-resolution separations of intact proteins with LC-TOF-MS using polymer monolithic columns
- 2008Errors involved in the Existing B-term Expressions for the Longitudinal Diffusion in Fully Porous Chromatographic Media. Part II: Experimental data in Packed Columns and Surface Diffusion Measurements
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article
A Methodology for the Estimation and Modelling of the Obstruction Factor in the Expression for Mesopore Diffusion in Reversed-Phase Liquid Chromatography Particles
Abstract
An experimental methodology for the determination of the obstruction factor in the expression for mesopore diffusion in Zorbax Eclipse Plus C-18 reversed-phase particles is proposed. The method uses peak parking experiments conducted on particles that were previously stripped of their stationary phase by flushing the column with trifluoroacetic acid at a temperature of 60 degrees C. Further using pure organic solvents as the mobile phase, any potential retention or surface diffusion effect is omitted. To avoid interference between the parked peaks and baseline disturbances typically occurring when switching on and off the flow, peak parking experiments were carried out in a set-up wherein two identical columns were used in parallel. This set-up allowed to maintain the flow through the detector at all times, by redirecting the flow from one column to the other during the peak parking experiments. Several tracer molecules (ionic and deuterated tracers) were compared and it was found that the use of deuterated molecules provides the best possible coverage of the accessible space of the mesopore volume. Interpreting the peak parking responses obtained with these tracers with a model based on the effective medium theory (EMT) subsequently provided an estimate of the value of the mesopore diffusion obstruction factor gamma(mp). Taking the well-established pore hindrance factor F(lambda) correction into account, the obtained experimental gamma(mp)- values are more in agreement with the tortuous and constricted diffusion paths one can expect in the void space within a structure resembling a monolithic skeleton with tetrahedral connectivity rather than in the void space formed by a packing of nanospheres. This is also more in line with the measured internal porosity values lying around epsilon(pz) =0.5, whereas a packing of nanospheres would rather correspond to an epsilon(pz) of 0.4. As such, the presented protocol provides a means to infer the internal mesopore structure of reversed-phase particles. (C) 2020 Elsevier B.V. All rights reserved.