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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Madeira, Pp
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Topics
Publications (6/6 displayed)
- 2013Analysis of amino acid-water interactions by partitioning in aqueous two-phase systems. I-Amino acids with non-polar side-chainscitations
- 2008Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systemscitations
- 2008"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) in PEG-salt and Ucon-salt aqueous two-phase systemscitations
- 2004Representation of liquid-liquid equilibria for polymer-salt aqueous two-phase systemscitations
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article
"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systems
Abstract
Distribution coefficients of randomly selected proteins were measured in aqueous two-phase systems (ATPSs) formed by different combinations of Dextran-75 (Dex), Ficoll-70, polyethylene glycol-8000 (PEG), hydroxypropyl starch-100 (PES), and Ucon50HB5100 (Ucon, a random copolymer of ethylene glycol and propylene glycol) at particular polymer concentrations, all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4. Most of the proteins in the PEG-Ucon system precipitated at the interface. In the other ATPSs, namely, PES-PEG, PES-Ucon, Ficoll-PEG, Ficoll-Ucon, and in Dex-PEG and Dex-Ucon described earlier the distribution coefficients for the proteins were correlated according to the solvent regression equation: In K-i = a(io) In K-o + b(io) where K-i and K-o are the distribution coefficients for any protein in the ith and oth two-phase systems. Coefficients a(io) and b(io) are constants, the values of which depend upon the particular compositions of the two-phase systems under comparison. (C) 2008 Published by Elsevier B.V.