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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Madeira, Pp
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Topics
Publications (6/6 displayed)
- 2013Analysis of amino acid-water interactions by partitioning in aqueous two-phase systems. I-Amino acids with non-polar side-chainscitations
- 2008Correlations between distribution coefficients of various biomolecules in different polymer/polymer aqueous two-phase systemscitations
- 2008"On the Collander equation": Protein partitioning in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systemscitations
- 2008Delta G(CH2) in PEG-salt and Ucon-salt aqueous two-phase systemscitations
- 2004Representation of liquid-liquid equilibria for polymer-salt aqueous two-phase systemscitations
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article
Delta G(CH2) as solvent descriptor in polymer/polymer aqueous two-phase systems
Abstract
Phase diagrams were determined for aqueous two-phase systems (ATPSs) formed by different paired combinations of Dextran (Dex-75), Ficoll-70, polyethylene glycol (PEG-8000), hydroxypropyl starch (PES-100), and Ucon50HB5100 (a random copolymer of ethylene glycol and propylene glycol) all containing 0.15 M NaCl in 0.01 M phosphate buffer, pH 7.4, at 23 degrees C. Partition coefficients of a series of dinitropherylated (DNP) amino acids with aliphatic side-chains were studied in all the ATPSs at particular polymer concentrations. Free energies of transfer of a methylene group between the coexisting phases, Delta G(CH2) were determined as measures of the difference between the hydrophobic character of the phases. Furthermore, partition coefficients of tryptophan (Trp) and its di- and tri-peptides and a set of p-nitrophenyl (NP)-monosaccharides were measured in all the two-phase systems, and the data obtained compared with the Delta G(CH2) values obtained in the systems. It was established that for eight out of 10 of two-phase systems of different polymer compositions the partition coefficients for Trp peptides correlate well with the Delta G(CH2) values. Similar correlations for NP-monosaccharides were valid for seven out of 10 two-phase systems. These observations indicate that the difference between the hydrophobic characters of the coexisting phases represented by the Delta G(CH2) value cannot be used as a single universal measure for comparison of the ATPSs of different polymer compositions.