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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Hokkanen, Ari
VTT Technical Research Centre of Finland
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (13/13 displayed)
- 2024Material engineering and application of hybrid biomimetic-de novo designed elastin-like polypeptidescitations
- 2024Respiratory rate monitoring with cellulose optical fiber
- 2022Optical properties of an organic-inorganic hybrid film made of regenerated cellulose doped with light-scattering TiO2 particlescitations
- 2022Hybrid films from cellulose nanomaterials—properties and defined optical patternscitations
- 2021Cellulose optical fiber
- 2021Cellulose optical fiber
- 2020Elastic and fracture properties of free-standing amorphous ALD Al2O3 thin films measured with bulge testcitations
- 2018Elastic and fracture properties of free-standing amorphous ALD Al2O3 thin films measured with bulge testcitations
- 2008Real-time analysis of PCR inhibition on microfluidic materialscitations
- 2007Use of brazing technique for manufacturing of high temperature fibre optical temperature and displacement transducercitations
- 2007Coating integrated optical fibres for monitoring of boiler heat transfer surfacescitations
- 2006Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchipcitations
- 2006Tunable hydrophilicity on a hydrophobic fluorocarbon polymer coating on siliconcitations
Places of action
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article
Design and fabrication of integrated solid-phase extraction-zone electrophoresis microchip
Abstract
Integrated solid-phase extraction-zone electrophoresis (SPE-ZE) device has been designed and fabricated on microchip. The structures were fabricated by using multiple layers of SU-8 polymer with a novel technique that enables easy alignment and high yield of the chips. SU-8 adhesive bonding has two major advantages: it enables bonding of high aspect ratio pillars and it results in fully SU-8 microchannels with uniform electrokinetic flow properties. The SPE-ZE device has a fluidic reservoir with 15:1 high aspect ratio pillars for bead filters that act as a SPE part in the chip structure. The separation unit is a 25 mm long electrophoresis channel starting from the outlet of SPE reservoir. Argon laser-induced fluorescence (LIF) detector was used to monitor simultaneously the SPE reservoir and the detection site at the end of the electrophoresis channel. Flow characteristics and electric field distributions were simulated with Femlab software. Fluorescein was used as the analyte for detecting the operational performance of the chip. Adsorption, bead rinsing, elution and detection were tested to verify functioning of the chip design.