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Howard, Shaun
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Publications (4/4 displayed)
- 2023New insight into degradation mechanisms of conductive and thermally resistant polyaniline filmscitations
- 2023Solvent-free Surface Modification of Milled Carbon Fiber using Resonant Acoustic Mixing
- 2020Lipid Nanodiscs via Ordered Copolymerscitations
- 2015Protic ionic liquids (PILs) nanostructure and physicochemical properties: development of high-throughput methodology for PIL creation and property screenscitations
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article
Lipid Nanodiscs via Ordered Copolymers
Abstract
A highly alternating copolymer composed of acrylic acid and styrene (AASTY) is synthesized with controlled radical polymerization by exploiting the reactivity ratios of the monomers to control the monomer sequence. The AASTY copolymers are effective solubilizers of cellular membranes and their embedded proteins,which improves structural characterization by single-particle cryo-electron microscopy (cryo-EM). These copolymers are promising tools for exploring detergent-free membrane protein solubilization and direct formation of native nanodiscs, facilitating structural and functional analysis of the mammalian proteome.