| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Croes, Kim
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 2016Assessment of estrogenic activity in PM10 air samples with the ERE-CALUX bioassaycitations
- 2001Study of Bolted Joints in Sandwich elements with Cementitious Composite Faces for Civil Engineering Applications
- 2001Design Procedure and Validation tests for a Composite-Concrete Pedestrian Bridge Prototype
- 2001Behaviour of Sandwich Panels with E-glass Fibre Reinforced Cementitious Faces under Repeated Loading
- 2001Testing of a Dowel Connection for a Bridge with a Concrete Deck and a Sandwich Panel Truss Structure
- 2000A new non-alkaline Cement Material Reinforced by Glass Fibres (IPC) for the Construction of Bridges
- 2000The use of a new cementitious glass fibre reinforced composite in sandwich structures for building applications
- 2000Modelling of a Modular Pedestrian Bridge composed of a Concrete Deck and a Truss Girder with IPC Sandwich Panels
- 2000Evaluation of fatigue and durability properties of E-glass reinforced phosphate cementitious composite
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article
Assessment of estrogenic activity in PM10 air samples with the ERE-CALUX bioassay
Abstract
<p>Endocrine disrupting chemicals represent a broad class of compounds, are widespread in the environment and can pose severe health effects. The objective of this study was to investigate the overall estrogen activating potential of PM10 air samples at an urban location with high traffic incidence in Flanders, using a human in vitro cell bioassay. PM10 samples (n = 36) were collected on glass fiber filters every six days between April 2013 and January 2014 using a high-volume sampler. Extraction was executed with a hexane/acetone mixture before analysis using a recombinant estrogen-responsive human ovarian carcinoma (BG1Luc4E2) cell line. In addition, several samples and procedural blanks were extracted with ultra-pure ethanol or acetonitrile to compare extraction efficiencies. Results were expressed as bioanalytical equivalents (BEQs) in femtogram 17β-estradiol equivalent (fg E2-Eq) per cubic meter of air. High fluctuations in estrogenic activity were observed during the entire sampling period, with mean and median BEQs of 50.7 and 35.9 fg E2-Eq m(-)(3), respectively. Estrogenic activity was measured in more than 70% of the samples and several sample extracts showed both high BEQs and high cytotoxicity, which could not be related to black carbon, PM10 or heavy metal concentrations. At this moment, it remains unclear which substances cause this toxicity, but comparison of results obtained with different extraction solvents indicated that acetone/hexane extracts contained more compounds that were cytotoxic and suppressive of responses than those extracted using ultra-pure ethanol. Although more research is needed, the use of a more polar extraction solvent seems to be advisable.</p>