• Astolfi, Laura
• Candito, Mariarita
• Mota, Carlos
• Azimi, Bahareh
• Lucena, Mónica
• Coltelli, Maria Beatrice
• Zavagna, Lorenzo
• Moroni, Lorenzo
• Ricci, Claudio
• Danti, Serena
• Berrettini, Stefano
• Anand, Shivesh
• Lazzeri, Andrea

Abstract

The tympanic membrane (TM), is a thin tissue lying at the intersection of the outer and the middle ear. TM perforations caused by traumas and infections often result in a conductive hearing loss. Tissue engineering has emerged as a promising approach for reconstructing the damaged TM by replicating the native material characteristics. In this regard, chitin nanofibrils (CN), a polysaccharide-derived nanomaterial, is known to exhibit excellent biocompatibility, immunomodulation and antimicrobial activity, thereby imparting essential qualities for an optimal TM regeneration. This work investigates the application of CN as a nanofiller for poly(ethylene oxide terephthalate)/poly(butylene terephthalate) (PEOT/PBT) copolymer to manufacture clinically suitable TM scaffolds using electrospinning and fused deposition modelling. The inclusion of CN within the PEOT/PBT matrix showed a three-fold reduction in the corresponding electrospun fiber diameters and demonstrated a significant improvement in the mechanical properties required for TM repair. Furthermore, in vitro biodegradation assay highlighted a favorable influence of CN in accelerating the scaffold degradation over a period of one year. Finally, the oto- and cytocompatibility response of the nanocomposite substrates corroborated their biological relevance for the reconstruction of perforated eardrums.

Topics

• Deposition
• nanocomposite
• impedance spectroscopy
• inclusion
• copolymer
• electrospinning
• biocompatibility