| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Goncalves, Mp
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (11/11 displayed)
- 2021Physicochemical and microstructural properties of composite edible film obtained by complex coacervation between chitosan and whey protein isolatecitations
- 2020Chemical/Color Stability and Rheological Properties of Cyanidin-3-Glucoside in Deep Eutectic Solvents as a Gateway to Design Task-Specific Bioactive Compoundscitations
- 2019Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogencitations
- 2015Alternative plasticizers for the production of thermo-compressed agar filmscitations
- 2015Electrospinning of agar/PVA aqueous solutions and its relation with rheological propertiescitations
- 2015Improving agar electrospinnability with choline-based deep eutectic solventscitations
- 2014Choline chloride based ionic liquid analogues as tool for the fabrication of agar films with improved mechanical propertiescitations
- 2013Ultrasound-assisted preparation of size-controlled chitosan nanoparticles: Characterization and fabrication of transparent biofilmscitations
- 2012Aggregation-induced conformational transitions in bovine beta-lactoglobulin adsorbed onto open chitosan structurescitations
- 2009Rheological properties of vaginal hydrophilic polymer gelscitations
- 2007Heat-induced gelation of beta-lactoglobulin at varying pH: Effect of tara gum on the rheological and structural properties of the gelscitations
Places of action
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article
Development of a disposable paper-based potentiometric immunosensor for real-time detection of a foodborne pathogen
Abstract
This work reports a new paper-based sensing platform and its application in a label-free potentiometric immunosensor for Salmonella typhimurium detection based on the blocking surface principle. A paper-based strip electrode was integrated with a filter paper pad which acted as a reservoir of the internal solution. The design offers a convenient platform for antibody immobilization and sampling, proving also that is a simple and affordable methodology to control an ionic flux through a polymer membrane. Two different immunosensing interfaces were assembled on the developed paper-strip electrode. The simplest interface relied on direct conjugation of the antibody to the polymer membrane and the second one resorted to an intermediate layer of a polyamidoamine dendrimer, with an ethylenediamine core from the fourth generation. Electrochemical impedance spectroscopy was used to assess the successive interface modification steps and the resulting analytical performance of both immunosensors was compared. For such, the potential shift derived from the blocking effect of the ionic flux caused by antigen-antibody conjugation was correlated with the logarithm of the Salmonella typhimurium concentration in the sample. In optimized conditions, a limit of detection of 5 cells mL(-1) was achieved. As a proof-of-concept, the proposed method was applied to apple juice samples, demonstrating to be a suitable prototype to be used in real scenarios in useful time ( < 1 h assay).