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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Guedes, Alexandra
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2024Highly Efficient and Magnetically Recyclable Non-Noble Metal Fly Ash-Based Catalysts for 4-Nitrophenol Reductioncitations
- 2024Graphitization: Microstructural and microtextural transformations of residual char from international coal combustion ashcitations
- 2024Graphitization: Microstructural and microtextural transformations of residual char from international coal combustion ashcitations
- 2023FoodSmarTag: An innovative dynamic labeling system based on pyranoflavylium-based colorimetric films for real-time monitoring of food freshnesscitations
- 2021Graphene@Metal Sulfide/Oxide Nanocomposites as Novel Photo-Fenton-like Catalysts for 4-Nitrophenol Degradationcitations
- 2020Hydrothermal Carbon/Carbon Nanotube Composites as Electrocatalysts for the Oxygen Reduction Reactioncitations
- 2018Electrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentagecitations
- 2018Photochromic polypropylene fibers based on UV-responsive silica@phosphomolybdate nanoparticles through melt spinning technologycitations
- 2018Petrographic and SEM/EDS characterization of bottom ash fractions obtained using magnetic separation equipment
- 2018Heteroatom-Doped Carbon Nanomaterials as Metal-Free Catalysts for the Reduction of 4-Nitrophenolcitations
- 2016Characterization of bottom ash of Pliocene lignite as ceramic composites raw material by petrographic, SEM/EDS and Raman microspectroscopical methodscitations
- 2014Tailored design of CoxMn1-xFe2O4 nanoferrites: a new route for dual control of size and magnetic propertiescitations
- 2014Gold nanoparticles decorated on Bingel-thiol functionalized multiwall carbon nanotubes as an efficient and robust catalystcitations
- 2012Multianalytical approaches to the characterisation of minerals associated with coals and the diagnosis of their potential risk by using combined instrumental microspectroscopic techniques and thermodynamic speciationcitations
- 2012Superparamagnetic MFe2O4 (M = Fe, Co, Mn) Nanoparticles: Tuning the Particle Size and Magnetic Properties through a Novel One-Step Coprecipitation Routecitations
Places of action
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article
Electrochemical genoassays on gold-coated magnetic nanoparticles to quantify genetically modified organisms (GMOs) in food and feed as GMO percentage
Abstract
The integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell Fe3O4@Au magnetic nanoparticles (MNPs) were used as the platform for the immobilization of event-specific Roundup Ready (RR) soybean and taxon-specific DNA sequences. Firstly, monodisperse Fe3O4 MNPs were synthesized by thermal decomposition and subsequently coated with a gold shell through reduction of Au(III) precursor on the surface of the MNPs in the presence of an organic capping agent This nanosupport exhibited high colloidal stability, average particle size of 10.2 +/- 1.3 rim, and spherical shape. The covalent immobilization of ssDNA probe onto the Au shell of the Fe3O4@Au MNPs was achieved through a self-assembled monolayer (SAM) created from mixtures of alkane thiols (6-mercapto-l-hexanol and mercaptohexanoic acid). The influence of the thiols ratio on the electrochemical performance of the resulting electrochemical genoassays was studied, and remarkably, the best analytical performance was achieved for a pure mercaptohexanoic acid SAM. Two quantification assays were designed; one targeting an RR sequence and a second targeting a reference soybean gene, both with a sandwich format for hybridization, signaling probes labelled with fluorescein isothiocyanate (FITC), enzymatic amplification and chronoamperometric detection at screen-printed carbon electrodes (SPCE). The magnetogenoassays exhibited linear ranges from 0.1 to 10.0 nM and from 0.1 to 5.0 nM with similar detection limits of 0.02 nM and 0.05 nM for the event-specific (RR) and the taxon-specific (lectin) targets, respectively. The usefulness of the approach was demonstrated by its application to detect genetically modified organisms (GMOs) in feed and food.