• Sarkar, Amrita
• Jolly, Pawan
• Martinez, Juana Reyes
• Heeran, Mel
• Formisano, Nello
• Flitsch, Sabine
• Estrela, Pedro
• Bowen, Christopher R.
• Laabei, Maisem
• Bhalla, Nikhil
• Taylor, John

Abstract

While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that last a few days, employing complex and expensive techniques. Therefore, innovative, simple, rapid and low-cost solutions to effectively reduce the burden of bacterial infections are urgently needed. Here we demonstrate a label-free sensor for fast bacterial detection based on metal–oxide–semiconductor field-effect transistors (MOSFETs). The electric charge of bacteria binding to the glycosylated gates of a MOSFET enables quantification in a straightforward manner. We show that the limit of quantitation is 1.9 × 105 CFU/ml with this simple device, which is more than 10,000-times lower than is possible with existing techniques such as matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF) on the same modified surfaces. Moreover, the measurements are extremely fast and the sensor can be mass produced at trivial cost as a tool for initial screening of pathogens.

Topics

• impedance spectroscopy
• surface
• semiconductor
• matrix-assisted laser desorption–ionisation
• spectrometry
• time-of-flight mass spectrometry