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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Hernández, S.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (8/8 displayed)
- 2016Heterogeneous distribution of B-site cations in BaZrxTi1−xO3 epitaxial thin films grown on (0 0 1) SrTiO3 by pulsed laser depositioncitations
- 2015Observation of room temperature photoluminescence from asymmetric CuGaO2/ Zno/ZnMgo multiple quantum well structurescitations
- 2014Optimization of alternative distillation sequences for natural gas sweeteningcitations
- 2014New strategies in laser processing of TCOs for light management improvement in thin-film silicon solar cells
- 2012Characterization of water uptake by organic coatings used for the corrosion protection of steel as determined from capacitance measurements
- 2009Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensorscitations
- 2007Bioaffinity platforms based on carbon-polymer biocomposites for electrochemical biosensingcitations
- 2007In situ DNA amplification with magnetic primers for the electrochemical detection of food pathogenscitations
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article
Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensors
Abstract
An immunoassay-based strategy for folic acid in vitamin-fortified milk with electrochemical detection using magneto sensors is described for the first time. Among direct and indirect competitive formats, best performance was achieved with an indirect competitive immunoassay. The immunological reaction for folic acid (FA) detection was performed, for the first time on the magnetic bead as solid support by the covalent immobilization of a protein conjugate BSA-FA on tosyl-activated magnetic bead. Further competition for the specific antibody between FA in the food sample and FA immobilized on the magnetic bead was achieved, followed by the reaction with a secondary antibody conjugated with HRP (AntiIgG-HRP). Then, the modified magnetic beads were easily captured by a magneto sensor made of graphite-epoxy composite (m-GEC) which was also used as the transducer for the electrochemical detection. The performance of the immunoassay-based strategy with electrochemical detection using magneto sensors was successfully evaluated using spiked-milk samples and compared with a novel magneto-ELISA based on optical detection. The detection limit was found to be of the order of μg l-1 (13.1 nmol l-1, 5.8 μg l-1) for skimmed milk. Commercial vitamin-fortified milk samples were also evaluated obtaining good accuracy in the results. This novel strategy offers great promise for rapid, simple, cost-effective and on-site analysis of biological and food samples. © 2008 Elsevier B.V. All rights reserved.