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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Fabiano, S.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 2023Improved Performance of Organic Thermoelectric Generators Through Interfacial Energeticscitations
- 2021Lactone backbone density in rigid electron-deficient semiconducting polymers enabling high n-type organic thermoelectric performancecitations
- 2021Lactone backbone density in rigid electron-deficient semiconducting polymers enabling high n-type organic thermoelectric performancecitations
- 2019Impact of Singly Occupied Molecular Orbital Energy on the n-Doping Efficiency of Benzimidazole-Derivativescitations
- 2018Air-Stable Benzimidazoline n-Type Dopants for Conductive Host Materials with Low Electron Affinities
- 2009Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensorscitations
- 2007Bioaffinity platforms based on carbon-polymer biocomposites for electrochemical biosensingcitations
- 2005The EpiLink record linkage software: presentation and results of linkage test on cancer registry files.citations
- 2003Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support.citations
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article
Immunoassay for folic acid detection in vitamin-fortified milk based on electrochemical magneto sensors
Abstract
An immunoassay-based strategy for folic acid in vitamin-fortified milk with electrochemical detection using magneto sensors is described for the first time. Among direct and indirect competitive formats, best performance was achieved with an indirect competitive immunoassay. The immunological reaction for folic acid (FA) detection was performed, for the first time on the magnetic bead as solid support by the covalent immobilization of a protein conjugate BSA-FA on tosyl-activated magnetic bead. Further competition for the specific antibody between FA in the food sample and FA immobilized on the magnetic bead was achieved, followed by the reaction with a secondary antibody conjugated with HRP (AntiIgG-HRP). Then, the modified magnetic beads were easily captured by a magneto sensor made of graphite-epoxy composite (m-GEC) which was also used as the transducer for the electrochemical detection. The performance of the immunoassay-based strategy with electrochemical detection using magneto sensors was successfully evaluated using spiked-milk samples and compared with a novel magneto-ELISA based on optical detection. The detection limit was found to be of the order of μg l-1 (13.1 nmol l-1, 5.8 μg l-1) for skimmed milk. Commercial vitamin-fortified milk samples were also evaluated obtaining good accuracy in the results. This novel strategy offers great promise for rapid, simple, cost-effective and on-site analysis of biological and food samples. © 2008 Elsevier B.V. All rights reserved.