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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Jesionowski, Teofil
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (24/24 displayed)
- 2025Unraveling the photocatalytic and antimicrobial performance of carbon-TiO2/lignin hybrid admixtures in sustainable cement compositescitations
- 2023Antimicrobial action and chemical and physical properties of CuO-doped engineered cementitious compositescitations
- 2023Creation of a 3D Goethite–Spongin Composite Using an Extreme Biomimetics Approachcitations
- 2023Spongin as a Unique 3D Template for the Development of Functional Iron-Based Composites using Biomimetic Approach In Vitrocitations
- 2023Carbon-modified TiO2 as a promising and efficient admixture for cementitious composites: A comprehensive study of photocatalytic, mechanical and structural propertiescitations
- 2022Novel Mesoporous Organosilicas with Task Ionic Liquids: Properties and High Adsorption Performance for Pb(II)citations
- 2021Tailor-made novel electrospun polystyrene/poly(D,L-lactide-co-glycolide) for oxidoreductases immobilization: Improvement of catalytic properties under extreme reaction conditionscitations
- 2021A novel microwave-assisted strategy to fabricate multifunctional photoactive titania-based heterostructures with enhanced activitycitations
- 20203D Chitin Scaffolds of Marine Demosponge Origin for Biomimetic Mollusk Hemolymph-Associated Biomineralization Ex-Vivocitations
- 2020The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removalcitations
- 2020Functionalization of 3D Chitinous Skeletal Scaffolds of Sponge Origin Using Silver Nanoparticles and Their Antibacterial Propertiescitations
- 2019Extreme biomimetics: Preservation of molecular detail in centimeter-scale samples of biological meshes laid down by spongescitations
- 2019Hydrothermal synthesis of multifunctional TiO2-ZnO oxide systems with desired antibacterial and photocatalytic propertiescitations
- 2019Dendrimer based theranostic nanostructures for combined chemo- and photothermal therapy of liver cancer cells in vitrocitations
- 2018Polydopamine grafted on an advanced Fe <inf>3</inf> O <inf>4</inf> /lignin hybrid material and its evaluation in biosensingcitations
- 2018GQDs-MSNs nanocomposite nanoparticles for simultaneous intracellular drug delivery and fluorescent imagingcitations
- 2018Cyclodextrin-based magnetic nanoparticles for cancer therapycitations
- 2017Lipase B from candida Antarctica immobilized on a silica-lignin matrix as a stable and reusable biocatalytic systemcitations
- 2016Lignosulfonate as a byproduct of wood pulp production: A potential precursor for the preparation of functional hybrid materialscitations
- 2015Extreme biomimetic approach for developing novel chitin-$GeO_{2}$ nanocomposites with photoluminescent propertiescitations
- 2015Solvothermal synthesis of hydrophobic chitin–polyhedral oligomeric silsesquioxane (POSS) nanocompositescitations
- 2014Synthesis of nanostructured chitina–hematite composites under extreme biomimetic conditionscitations
- 2012Structural Characterisation of ZnO Particles Obtained by the Emulsion Precipitation Methodcitations
- 2012Influence of Selected Alkoxysilanes on Dispersive Properties and Surface Chemistry of Titanium Dioxide and TiO2–SiO2 Composite Materialcitations
Places of action
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article
Tailor-made novel electrospun polystyrene/poly(D,L-lactide-co-glycolide) for oxidoreductases immobilization: Improvement of catalytic properties under extreme reaction conditions
Abstract
Immobilized enzymes find applications in many areas such as pharmacy, medicine, food production and environmental protection. However, protecting these biocatalysts against harsh reaction conditions and retaining their enzymatic activity even after several biocatalytic cycles are major challenges. Properly selected supports and type of surface modifier therefore seem to be crucial for achieving high retention of catalytic activity of immobilized biomolecules. Here we propose production of novel composite electrospun fibers from polystyrene/poly(D,L-lactide-co-glycolide) (PS/PDLG) and its application as a support for immobilization of oxidoreductases such as alcohol dehydrogenase (ADH) and laccase (LAC). Two strategies of covalent binding, (i) (3-aminopropyl)triethoxysilane (APTES) with glutaraldehyde (GA and (ii) polydopamine (PDA), were applied to attach oxidoreductases to PS/PDLG. The average fiber diameter was shown to increase from 1.252 µm to even 3.367 µm after enzyme immobilization. Effective production of PS/PDLG fibers and biomolecule attachment were confirmed by Fourier transform infrared spectroscopy analysis. The highest substrate conversion efficiency was observed at pH 6.5 and 5 for ADH and LAC, respectively, and at 25 °C for enzymes attached using the APTES+GA approach. Improvement of enzyme stabilization at high temperatures was confirmed in that relative activities of enzymes immobilized onto PS/PDLG fibers were over 20% higher than those of the free biomolecules, and enzyme leaching from the support using acetate and MES buffers was below 10 mg/g.