| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Caruso, Frank
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (16/16 displayed)
- 2023Protease-Responsive Hydrogel Microparticles for Intradermal Drug Deliverycitations
- 2020Protein Component of Oyster Glycogen Nanoparticlescitations
- 2019The future of layer-by-layer assembly: A tribute to ACS Nano associate editor Helmuth Möhwaldcitations
- 2019Glycogen as a building block for advanced biological materialscitations
- 2018Metal-Organic Frameworks for Cell and Virus Biologycitations
- 2015Surface-Confined Amorphous Films from Metal-Coordinated Simple Phenolic Ligandscitations
- 2015Nanoporous Metal-Phenolic Particles as Ultrasound Imaging Probes for Hydrogen Peroxide.citations
- 2014Tuning particle biodegradation through polymer-peptide blend compositioncitations
- 2012Tailoring the Chain Packing in Ultrathin Polyelectrolyte Films Formed by Sequential Adsorptioncitations
- 2012Synthesis and functionalization of nanoengineered materials using click chemistrycitations
- 2012Engineering cellular degradation of multilayered capsules through controlled cross-linkingcitations
- 2011Nanoengineered films via surface-confined continuous assembly of polymerscitations
- 2009Cholesterol-mediated anchoring of enzyme-loaded liposomes within disulfide-stabilized polymer carrier capsulescitations
- 2009Tuning the formation and degradation of layer-by-layer assembled polymer hydrogel microcapsulescitations
- 2009Stabilization and functionalization of polymer multilayers and capsules via thiol-ene click chemistrycitations
- 2005Optical Properties of Nanoparticle-based Metallodielectric Inverse Opalscitations
Places of action
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article
Cholesterol-mediated anchoring of enzyme-loaded liposomes within disulfide-stabilized polymer carrier capsules
Abstract
<p>Polymer capsules containing multiple liposomes, termed capsosomes, are a promising new concept toward the design of artificial cells. Herein, we report on the fundamental aspects underpinning the assembly of capsosomes. A stable and high loading of intact liposomal cargo into a polymer film was achieved by non-covalently sandwiching the liposomes between a tailor-made cholesterol-modified poly(l-lysine) (PLL<sub>c</sub>) precursor layer and a poly(methacrylic acid)-co-(cholesteryl methacrylate) (PMA<sub>c</sub>) capping layer. The film assembly, optimized on planar surfaces, was successfully transferred onto colloidal substrates, and a polymer membrane was subsequently assembled by the alternating adsorption of poly(N-vinyl pyrrolidone) (PVP) and thiol-modified poly(methacrylic acid) (PMA<sub>SH</sub>) onto the pre-adsorbed layer of liposomes. Upon removal of the silica template, stable capsosomes encapsulating the enzyme luciferase or β-lactamase within their liposomal sub-compartments were obtained at both assembly (pH 4) and physiological conditions (pH 7.4). Excellent retention of the liposomes and the enzymatic cargo within the polymer carrier capsules was observed for up to 14 days. These engineered capsosomes are particularly attractive as autonomous microreactors, which can be utilized to repetitively add smaller reactants to cause successive distinct reactions within the capsosomes and simultaneously release the products to the surrounding environment, bringing these systems one step closer toward constructing artificial cells.</p>