| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Górski, Łukasz
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2017Phosphorothioated oligonucleotides as receptor layers in electrochemical detection of uranyl cationcitations
- 2016Electrochemical Determination of Hg2+ Using Electrodes Modified with Peptide Nucleic Acidcitations
- 2015Electrochemical oligonucleotide-based biosensor for the determination of lead ioncitations
- 2013Development of silicon-based electrochemical transducerscitations
- 2013Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicatorcitations
- 2009Influence of inner transducer properties on EMF response and stability of solid-contact anion-selective membrane electrodes based on metalloporphyrin ionophorescitations
- 2004Polymeric membrane electrodes with enhanced fluoride selectivity using Zr(IV)-porphyrins functioning as neutral carrierscitations
Places of action
| Organizations | Location | People |
|---|
article
Electrochemical oligonucleotide-based biosensor for the determination of lead ion
Abstract
The possibility of utilization of gold electrodes modified with short guanine-rich ssDNA probes for determination of Pb2 + was examined. Interaction between guanine residues and lead ion followed by formation of G-quadruplex structures was confirmed by electrochemical impedance spectroscopy investigations. An external cationic redox label, methylene blue, was employed in voltammetric measurements for analytical signal generation. It was shown that due to the G-quadruplex formation, the oligonucleotides in the recognition layer fold, which enhances the electron transfer between methylene blue and the electrode surface. The MB current signal rises proportionally to the lead ion concentration in the range from 0.05 to 1 μmol/L. The developed biosensor demonstrated high selectivity towards Pb2 + ion, with only minor response towards interfering metal cations. The calculated limit of detection was of 34.7 nmol/L. The utilization of the biosensor for Pb2 + determination in real samples of water was also tested.