| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Ziółkowski, Robert
in Cooperation with on an Cooperation-Score of 37%
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Publications (5/5 displayed)
- 2019Directly-thiolated graphene based electrochemical sensor for Hg(II) ioncitations
- 2017Graphene Nanoplatelets for Screen-Printed Nonenzymatic Voltammetric H2O2 Sensorscitations
- 2015Electrochemical oligonucleotide-based biosensor for the determination of lead ioncitations
- 2013Development of silicon-based electrochemical transducerscitations
- 2013Oligonucleotide-Based Electrochemical Biosensor for Hg2+ Using Methylene Blue as a Redox Indicatorcitations
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article
Electrochemical oligonucleotide-based biosensor for the determination of lead ion
Abstract
The possibility of utilization of gold electrodes modified with short guanine-rich ssDNA probes for determination of Pb2 + was examined. Interaction between guanine residues and lead ion followed by formation of G-quadruplex structures was confirmed by electrochemical impedance spectroscopy investigations. An external cationic redox label, methylene blue, was employed in voltammetric measurements for analytical signal generation. It was shown that due to the G-quadruplex formation, the oligonucleotides in the recognition layer fold, which enhances the electron transfer between methylene blue and the electrode surface. The MB current signal rises proportionally to the lead ion concentration in the range from 0.05 to 1 μmol/L. The developed biosensor demonstrated high selectivity towards Pb2 + ion, with only minor response towards interfering metal cations. The calculated limit of detection was of 34.7 nmol/L. The utilization of the biosensor for Pb2 + determination in real samples of water was also tested.