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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Behrends, Jan
University of Freiburg
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (19/19 displayed)
- 2023Photogeneration of spin quintet triplet–triplet excitations in DNA-assembled pentacene stackscitations
- 2023Photogeneration of Spin Quintet Triplet–Triplet Excitations in DNA-Assembled Pentacene Stacks
- 2023Photogeneration of Spin Quintet Triplet-Triplet Excitations in DNA-Assembled Pentacene Stacks.
- 2022The fast and the capacious ; a [Ni(Salen)]‐TEMPO redox‐conducting polymer for organic batteries
- 2022Spins at work: probing charging and discharging of organic radical batteries by electron paramagnetic resonance spectroscopy
- 2022Deoxyribonucleic Acid Encoded and Size-Defined π-Stacking of Perylene Diimidescitations
- 2022Deoxyribonucleic Acid Encoded and Size-Defined π-Stacking of Perylene Diimides
- 2022Deoxyribonucleic Acid Encoded and Size-Defined π-Stacking of Perylene Diimides.
- 2021Spin–spin interactions and spin delocalisation in a doped organic semiconductor probed by EPR spectroscopycitations
- 2020Expedient paramagnetic properties of surfactant-free plasmonic silicon-based nanoparticles
- 2020Correlated donor/acceptor crystal orientation controls photocurrent generation in all-polymer solar cellscitations
- 2018Reaction of porphyrin-based surface-anchored metal-organic frameworks to prolonged illumination
- 2018Reaction of porphyrin-based surface-anchored metal–organic frameworks caused by prolonged illumination
- 2016Triplet excitons as sensitive spin probes for structure analysis of extended defects in microcrystalline silicon
- 2006High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation.citations
- 2004Ion channel drug discovery and research: the automated Nano-Patch-Clamp technology.citations
- 2003Microstructured apertures in planar glass substrates for ion channel research.citations
- 2003High quality ion channel analysis on a chip with the NPC technology.citations
- 2002Whole cell patch clamp recording performed on a planar glass chip.citations
Places of action
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article
High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation.
Abstract
Microstructured planar substrates have been shown to be suitable for patch clamp recording from both whole cells and isolated patches of membrane, as well as for measurements from planar lipid bilayers. Here, we further explore this technology with respect to high-resolution, low noise single-channel recording. Using solvent-free lipid bilayers from giant unilamellar vesicles obtained by electro-swelling, we recorded channels formed by the peptaibol alamethicin, a well-studied model system for voltage-dependent channels, focusing on the transient dynamics of single-channel formation upon application of a voltage step. With our setup, we were able to distinctly resolve dwell times well below 100 mus and to perform a thorough statistical analysis of alamethicin gating. Our results show good agreement with models that do not rely on the existence of non-conducting preaggregate states. Microstructured apertures in glass substrates appear promising with respect to future experiments on cellular ion channels reconstituted in suspended lipid membranes.