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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Fawzy, Amr
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (23/23 displayed)
- 2024Effect of inositol hexaphosphate acid versus polyacrylic acid on dentin properties and adhesion of a self-adhesive restorative material to dentincitations
- 2024The Synergistic Effect of High Intensity Focused Ultrasound on In-vitro Remineralization of Tooth Enamel by Calcium Phosphate Ion Clusterscitations
- 2024Flowable resin-based composites modified with chlorhexidine-loaded mesoporous silica nanoparticles induce superior antibiofilm properties
- 2022Cytotoxicity and antimicrobial efficiency of ZrO2 nanoparticles reinforced 3D printed resinscitations
- 2022Development of 3D printed dental resin nanocomposite with graphene nanoplatelets enhanced mechanical properties and induced drug-free antimicrobial activity.citations
- 2021Development of 3D printed resin reinforced with modified ZrO2 nanoparticles for long-term provisional dental restorationscitations
- 2021Formulation of pH-sensitive chlorhexidine-loaded/mesoporous silica nanoparticles modified experimental dentin adhesivecitations
- 2020The Effect of Cross-linking Efficiency of Drug-Loaded Novel Freeze Gelated Chitosan Templates for Periodontal Tissue Regenerationcitations
- 2020Effect of acid etching on dentin bond strength of ultra-mild self-etch adhesivescitations
- 2020Properties of a modified quaternary ammonium silane formulation as a potential root canal irrigant in endodonticscitations
- 2020In vitro bonding performance of modern self-adhesive resin cements and conventional resin-modified glass ionomer cements to prosthetic substratescitations
- 2019Resin-based dental composites for tooth filling
- 2019Macrophage response and surface analysis of dental cementum after treatment with high intensity focused ultrasoundcitations
- 2019An in vitro study of a novel quaternary ammonium silane endodontic irrigantcitations
- 2019Co-blend application mode of bulk fill composite resincitations
- 2018In vitro assessment of ribose modified two-step etch-and-rinse dentine adhesivecitations
- 2017Proanthocyanidins-Loaded Nanoparticles Enhance Dentin Degradation Resistancecitations
- 2017Potentiating the antibacterial effect of silver nanospheres by surface-capping with chlorhexidine gluconatecitations
- 2015Characterization of antibacterial and adhesion properties of chitosan-modified glass ionomer cementcitations
- 2012Riboflavin as a dentin crosslinking agentcitations
- 2009Effect acidic and alkaline/heat treatments on the bond strength of different luting cements to commercially pure titaniumcitations
- 2009Tensile bond strength of immediately repaired anterior microfine hybrid restorative composite using nontrimmed hourglass specimens
- 2008Effect of surface treatments on the tensile bond strength of repaired water-aged anterior restorative micro-fine hybrid resin compositecitations
Places of action
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article
Macrophage response and surface analysis of dental cementum after treatment with high intensity focused ultrasound
Abstract
<p>Objective: To investigate effects of HIFU on macrophage phenotype, surface micro-topography and nano-scale surface mechanical properties of dental cementum. Materials and methods: Root discs (2 mm thickness) were cut apical to CEJ and sectioned into quadrants. HIFU setup with bowl-shaped piezo ceramic transducer submerged in a water tank was used for exposure on each specimen for 15 s, 30 s or 60 s. The specimens of the control group were left without any HIFU exposure. HIFU was generated with a continuous sinusoidal wave of 120Vpp amplitude, 250 KHZ resonance-frequency and highest ultrasonic pressure of ∼10 bar at the focus. Specimens for SEM were viewed, and micro-topography characterization performed, using AFM and Ra parameter and surface area (SA) calculated by specialized SPM surface analysis software. For nano-indentation testing, experiments were carried out using AFM. Macrophage cell isolation and culturing was performed on cementum to receive the HIFU treatment at different time periods. Raman spectroscopy were scanned to create spectra perpendicular to the cementum substrate to analyze generation of standard spectra for Raman intensity ratio of hydroxyapatite normalized to the peaks ν<sub>1</sub> 960 cm<sup>−1</sup>. Data was expressed as means ± standard deviations and analyzed by one-way ANOVA in term of Ra, SA, H and E<sub>r</sub>. Different points for fluorescence intensity ratio were analyzed by Raman using Wilcoxon rank sum test. Results: HIFU exposure at 60 s removed the smear layer and most of cementum appeared smoothened. AFM characterisation, showed a slight decrease in the irregularity of the surface as exposure time increased. Intact macrophages can be identified in control and all experimental HIFU groups. The level of fluorescence for the control and HIFU 15 and 30 s were low as compared to HIFU 60 s. Conclusion: If HIFU can be successfully implemented, it may be a possible alternative to current methods used in periodontal therapy to achieve smooth root surfaces.</p>