| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Werner, Carsten
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (45/45 displayed)
- 202375 years of polymer research in Dresden
- 2023Discriminant Principal Component Analysis of ToF-SIMS Spectra for Deciphering Compositional Differences of MSC-Secreted Extracellular Matricescitations
- 2023Hydrogel-Coated Nanonet-Based Field-Effect Transistors for SARS-CoV-2 Spike Protein Detection in High Ionic Strength Samples
- 2023Dynamic matrices with DNA-encoded viscoelasticity for cell and organoid culturecitations
- 2022Microenvironment-mediated cancer dormancy: Insights from metastability theorycitations
- 2022Real-Time Monitoring of Blood Parameters in the Intensive Care Unit: State-of-the-Art and Perspectivescitations
- 2021Hemocompatible Electrochemical Sensors for Continuous Monitoring of Blood Parameterscitations
- 2021Bioresponsive starPEG-heparin hydrogel coatings on vascular stents for enhanced hemocompatibilitycitations
- 2020Protein Component of Oyster Glycogen Nanoparticlescitations
- 2019Highly conductive, stretchable, and cell‐adhesive hydrogel by nanoclay dopingcitations
- 2019Impact of oral astringent stimuli on surface charge and morphology of the protein-rich pellicle at the tooth–saliva interphasecitations
- 2019Fabrication of multifunctional titanium surfaces by producing hierarchical surface patterns using laser based ablation methodscitations
- 2019Temperature-Induced Mechanomodulation of Interpenetrating Networks of Star Poly(ethylene glycol)-Heparin and Poly(N-isopropylacrylamide)citations
- 2019Springtail-inspired triangular laser-induced surface textures on metals using mhz ultrashort pulsescitations
- 2018Analyzing the antiseptic capacity of silver-functionalized poly(ethylene glycol)-heparin hydrogels after human whole blood exposurecitations
- 2017Solvent-assisted micromolding of biohybrid hydrogels to maintain human hematopoietic stem and progenitor cells ex vivocitations
- 2017Cell-instructive starPEG-heparin-collagen composite matricescitations
- 2017Forbidden Chemistrycitations
- 2017Macromolecular crowding for tailoring tissue-derived fibrillated matricescitations
- 2016A hyperbranched dopamine-containing PEG-based polymer for the inhibition of α-synuclein fibrillationcitations
- 2015Thermo-responsive cell culture carriers based on poly(vinyl methyl ether) - The effect of biomolecular ligands to balance cell adhesion and stimulated detachment
- 2015Thermo-responsive cell culture carriers based on poly(Vinyl Methyl ether) - the effect of biomolecular ligands to balance cell adhesion and stimulated detachment.citations
- 2014Influence of Individual Phospholipids on the Physical Properties of Oil-Based Suspensionscitations
- 2013The impact of lecithin on rheology, sedimentation and particle interactions in oil-based dispersionscitations
- 2013Permanent surface modification by electron-beam-induced grafting of hydrophilic polymers to PVDF membranescitations
- 2013On the use of electrokinetics for unraveling charging and structure of soft planar polymer filmscitations
- 2013ECM modulated early kidney development in embryonic organ culturecitations
- 2012Surface modification of cell culture carrierscitations
- 2012Polymeric biomaterials for stem cell bioengineeringcitations
- 2011Biokompatible und bioaktive polymere Beschichtungencitations
- 2011Immobilization of Bacillus licheniformis α-amylase onto reactive polymer filmscitations
- 2011Enzyme immobilization on reactive polymer filmscitations
- 2011Modulating Biofunctional starPEG Heparin Hydrogels by Varying Size and Ratio of the Constituentscitations
- 2011Electron beam immobilization of functionalized poly(vinyl methyl ether) thin films on polymer surfaces - Towards stimuli responsive coatings for biomedical purposescitations
- 2011Enzymes for antifouling strategiescitations
- 2010The role of the interplay between polymer architecture and bacterial surface properties on the microbial adhesion to polyoxazoline-based ultrathin filmscitations
- 2010Studying the influence of chemical structure on the surface properties of polymer filmscitations
- 2010Hydroxide and hydronium ion adsorption - A surveycitations
- 2009Charging and structure of zwitterionic supported bilayer lipid membranes studied by streaming current measurements, fluorescence microscopy, and attenuated total reflection Fourier transform infrared spectroscopycitations
- 2009Temperature dependent physicochemical properties of poly(N- isopropylacrylamide-co-N-(1-phenylethyl) acrylamide) thin filmscitations
- 2009Electrostatic stretching of grafted maleic acid copolymer chainscitations
- 2004In vitro blood compatibility of polymeric biomaterials through covalent immobilization of an amidine derivativecitations
- 2002Characterization of oxide layers on Ti6Al4V and titanium by streaming potential and streaming current measurementscitations
- 2001Electrokinetic Measurements Reveal Interfacial Charge at Polymer Films Caused by Simple Electrolyte Ionscitations
- 2000Analiza povrsine vlaken z mikroskopijo atomskih sil (AFM)
Places of action
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article
Macromolecular crowding for tailoring tissue-derived fibrillated matrices
Abstract
<p>Tissue-derived fibrillated matrices can be instrumental for the in vitro reconstitution of multiphasic extracellular microenvironments. However, despite of several advantages, the obtained scaffolds so far offer a rather narrow range of materials characteristics only. In this work, we demonstrate how macromolecular crowding (MMC) – the supplementation of matrix reconstitution media with synthetic or natural macromolecules in ways to create excluded volume effects (EVE) – can be employed for tailoring important structural and biophysical characteristics of kidney-derived fibrillated matrices. Porcine kidneys were decellularized, ground and the obtained extracellular matrix (ECM) preparations were reconstituted under varied MMC conditions. We show that MMC strongly influences the fibrillogenesis kinetics and impacts the architecture and the elastic modulus of the reconstituted matrices, with diameters and relative alignment of fibrils increasing at elevated concentrations of the crowding agent Ficoll400, a nonionic synthetic polymer of sucrose. Furthermore, we demonstrate how MMC modulates the distribution of key ECM molecules within the reconstituted matrix scaffolds. As a proof of concept, we compared different variants of kidney-derived fibrillated matrices in cell culture experiments referring to specific requirements of kidney tissue engineering approaches. The results revealed that MMC-tailored matrices support the morphogenesis of human umbilical vein endothelial cells (HUVECs) into capillary networks and of murine kidney stem cells (KSCs) into highly branched aggregates. The established methodology is concluded to provide generally applicable new options for tailoring tissue-specific multiphasic matrices in vitro. Statement of Significance Tissue-derived fibrillated matrices can be instrumental for the in vitro reconstitution of multiphasic extracellular microenvironments. However, despite of several advantages, the obtained scaffolds so far offer a rather narrow range of materials characteristics only. Using the kidney matrix as a model, we herein report a new approach for tailoring tissue-derived fibrillated matrices by means of macromolecular crowding (MMC), the supplementation of reconstitution media with synthetic or natural macromolecules. MMC-modulation of matrix reconstitution is demonstrated to allow for the adjustment of fibrillation kinetics and nano-architecture, fiber diameter, alignment, and matrix elasticity. Primary human umbilical vein endothelial cells (HUVEC) and murine kidney stem cells (KSC) were cultured within different variants of fibrillated kidney matrix scaffolds. The results showed that MMC-tailored matrices were superior in supporting desired morphogenesis phenomena of both cell types.</p>