| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Müller, Ralph
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2024GaAs//CuInGaSe-Based Multijunction Solar Cells with 30% Efficiency Under Low Concentrated Sunlight
- 2022Long-term mechanical loading is required for the formation of 3D bioprinted functional osteocyte bone organoidscitations
- 2022Integrated Solar-Driven Device with a Front Surface Semitransparent Catalysts for Unassisted CO2 Reductioncitations
- 2021Nano‐3D‐printed photochromic micro‐objectscitations
- 2020Silicon-Based Monolithic Triple-Junction Solar Cells with Conversion Efficiency >34%
- 2018An automated step-wise micro-compression device for 3D dynamic image-guided failure assessment of bone tissue on a microstructural level using time-lapsed tomographycitations
- 2017On the determination of the contact resistivity for passivating contacts using 3D simulations
- 2015Effect of fetal bovine serum on mineralization in silk fibroin scaffoldscitations
- 2015Ion-implanted laser-annealed p+ and n+ regions. A potential solution for industrially feasible high-efficiency n-type interdigitated back-contact solar cellscitations
- 2013A quantitative framework for the 3D characterization of the osteocyte lacunar systemcitations
- 2011Analysis of sintered polymer scaffolds using concomitant synchrotron computed tomography and in situ mechanical testingcitations
- 2003Tomography studies of human foreskin fibroblasts on polymer yarnscitations
Places of action
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article
Effect of fetal bovine serum on mineralization in silk fibroin scaffolds
Abstract
Fetal bovine serum (FBS) is a common media supplement used in tissue engineering (TE) cultures. The chemical composition of FBS is known to be highly variable between different brands, types or batches and can have a significant impact on cell function. This study investigated the influence of four different FBS types in osteogenic or control medium on mineralization of acellular and cell-seeded silk fibroin (SF) scaffolds. In bone TE, mineralized tissue is considered as the final product of a successful cell culture. Calcium assays and micro-computed tomography scans revealed spontaneous mineralization on SF scaffolds with certain FBS types, even without cells present. In contrast, cell-mediated mineralization was found under osteogenic conditions only. Fourier transform infrared spectroscopy analysis demonstrated a similar ion composition of the mineralization present in scaffolds, whether cell-mediated or spontaneous. These results were confirmed by scanning electron microscopy. This study shows clear evidence for the influence of FBS type on mineralization on SF scaffolds. The suitability of FBS medium supplementation in TE studies is highly questionable with regard to reproducibility of studies and comparability of obtained results. For future TE studies, alternatives to conventional FBS such as defined FBS or serum-free media should be considered, as suggested decades ago.