| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Pereira, Cm
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (12/12 displayed)
- 2022Molecularly imprinted polymer as a synthetic antibody for the biorecognition of hazelnut Cor a 14-allergencitations
- 2021A Disposable Saliva Electrochemical MIP-Based Biosensor for Detection of the Stress Biomarker α-Amylase in Point-of-Care Applicationscitations
- 2018Electrodeposition of an ultrathin TiO2 coating using a deep eutectic solvent based on choline chloridecitations
- 2017Electrodeposition of Mn and Mn-Sn Alloy Using Choline Chloride-Based Ionic Liquidscitations
- 2017Zinc Electrodeposition from deep eutectic solvent containing organic additivescitations
- 2017Electrodeposition of Co and Co composites with carbon nanotubes using choline chloride-based ionic liquidscitations
- 2017Electrochemical detection of cardiac biomarker myoglobin using polyphenol as imprinted polymer receptorcitations
- 2016Metal cation sorption ability of immobilized and reticulated chondroitin sulfate or fucoidan through a sol-gel crosslinking schemecitations
- 2015Influence of Amines on the Electrodeposition of Zn-Ni Alloy from a Eutectic-Type Ionic Liquidcitations
- 2015Polymer composite materials modified with nano-oxides and phosphinates hybrid flame retardant systemscitations
- 2011Preparation and characterization of DNA films using oleylamine modified Au surfacescitations
- 2009Dielectric Relaxation and Optical Transmittance of PVC Membranes Modified by Nematic Liquid Crystal
Places of action
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article
Electrochemical detection of cardiac biomarker myoglobin using polyphenol as imprinted polymer receptor
Abstract
An electrochemical biosensor was developed by merging the features of Molecular Imprinting technique and Screen-Printed Electrode (SPE) for the simple and fast screening of cardiac biomarker myoglobin (Myo) in point-of-care (POC). The MIP artificial receptor for Myo was prepared by electrooxidative polymerization of phenol (Ph) on a AuSPE in the presence of Myo as template molecule. The choice of the most effective protein extraction procedure from the various extraction methods tested (mildly acidic/basic solutions, pure/mixed organic solvents, solutions containing surfactants and enzymatic digestion methods), and the optimization of the thickness of the polymer film was carefully undertaken in order to improve binding characteristics of Myo to the imprinted polymer receptor and increase the sensitivity of the MIP biosensor. The film thickness was optimized by adjusting scan rate and the number of cycles during cyclic voltammetric electropolymerization of Ph. The thickness of the polyphenol nanocoating of only few nanometres (similar to 4.4 nm), and similar to the protein diameter, brought in significant improvements in terms of sensor sensitivity. The binding affinity of MIP receptor film was estimated by fitting the experimental data to Freundlich isotherm and a similar to 8 fold increase in the binding affinity of Myo to the imprinted polymer (K-F = 0.119 +/- 0.002 ng(-1) mL) when compared to the non- imprinted polymer (K-F = 0.015 +/- 0.002 ng(-1) mL) which demonstrated excellent (re) binding affinity for the imprinted protein. The incubation of the Myo MIP receptor modified electrode with increasing concentration of protein (from 0.001 ng mL(-1) to 100 mu g mL(-1)) resulted in a decrease of the ferro/ferricyanide redox current. LODs of 2.1 and 14 pg mL(-1) were obtained from calibration curves built in neutral buffer and diluted artificial serum, respectively, using SWV technique, enabling the detection of the protein biomarker at clinically relevant levels. The prepared MIP biosensor was applied to the determination of Myo spiked serum samples with satisfactory results.