| People | Locations | Statistics |
|---|---|---|
| Naji, M. |
| |
| Motta, Antonella |
| |
| Aletan, Dirar |
| |
| Mohamed, Tarek |
| |
| Ertürk, Emre |
| |
| Taccardi, Nicola |
| |
| Kononenko, Denys |
| |
| Petrov, R. H. | Madrid |
|
| Alshaaer, Mazen | Brussels |
|
| Bih, L. |
| |
| Casati, R. |
| |
| Muller, Hermance |
| |
| Kočí, Jan | Prague |
|
| Šuljagić, Marija |
| |
| Kalteremidou, Kalliopi-Artemi | Brussels |
|
| Azam, Siraj |
| |
| Ospanova, Alyiya |
| |
| Blanpain, Bart |
| |
| Ali, M. A. |
| |
| Popa, V. |
| |
| Rančić, M. |
| |
| Ollier, Nadège |
| |
| Azevedo, Nuno Monteiro |
| |
| Landes, Michael |
| |
| Rignanese, Gian-Marco |
|
Gupta, V.
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (9/9 displayed)
- 2023An Unconventional Route for Synthesis and Solid-State Processing of Low-Entangled Ultra-High Molecular Weight Isotactic Polypropylenecitations
- 2021Association of MC4R (rs17782313) gene polymorphism with obesity measures in Western India.citations
- 2020Investigation of hysteresis in hole transport layer free metal halide perovskites cells under dark conditionscitations
- 2017Ruthenium based metallopolymer grafted reduced graphene oxide as a new hybrid solar light harvester in polymer solar cells.citations
- 20163D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatographycitations
- 2016Nickel(II) tetra-aminophthalocyanine modified MWCNTs as potential nanocomposite materials for the development of supercapacitorscitations
- 2014Shock-induced plasticity and the Hugoniot elastic limit in copper nano films and rodscitations
- 2008Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A from Mycobacterium tuberculosis.citations
- 2008Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of bacterioferritin A from Mycobacterium tuberculosiscitations
Places of action
| Organizations | Location | People |
|---|
article
3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography
Abstract
The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9 mm I.D. ? 600 mm long) was produced within a 5 ? 30 ? 30 mm titanium alloy (Ti?6Al?4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-<em>co</em>-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. Peak capacities of 69?76 (for 6?8 proteins respectively) were observed during isothermal separation of proteins at 44 ?C which were further increased to 73?77 using a thermal step gradient with programmed temperature from 60 ?C to 35 ?C using an in-house built direct-contact heater/cooler platform based upon matching sized Peltier thermoelectric modules. Rapid temperature gradients were possible due to direct-contact between the planar metal column and the Peltier module, and the high thermal conductivity of the titanium column as compared to a similar stainless steel printed column. The separation of peptides released from a digestion of <em>E.coli</em> was also achieved in less than 35 min with ca. 40 distinguishable peaks at 210 nm.