• Ribera, Javier
• Schwarze, Francis W. M. R.
• Martinez, Yolanda

Abstract

<jats:sec><jats:title>Abstract</jats:title><jats:p><jats:italic>Trichoderma</jats:italic> spp. are a genus of well-known fungi that promote healthy growth and modulate different functions in plants, as well as protect against various plant pathogens. The application of <jats:italic>Trichoderma</jats:italic> and its propagules as a biological control method can therefore help to reduce the use of chemical pesticides and fertilizers in agriculture. This review critically discusses and analyzes groundbreaking innovations over the past few decades of biotechnological approaches to prepare active formulations containing <jats:italic>Trichoderma</jats:italic>. The use of various carrier substances is covered, emphasizing their effects on enhancing the shelf life, viability, and efficacy of the final product formulation. Furthermore, the use of processing techniques such as freeze drying, fluidized bed drying, and spray drying are highlighted, enabling the development of stable, light-weight formulations. Finally, promising microencapsulation techniques for maximizing the performance of <jats:italic>Trichoderma</jats:italic> spp. during application processes are discussed, leading to the next-generation of multi-functional biological control formulations.</jats:p></jats:sec><jats:sec><jats:title>Key points</jats:title><jats:p>• <jats:italic>The development of carrier substances to encapsulate Trichoderma propagules is highlighted.</jats:italic></jats:p><jats:p>• <jats:italic>Advances in biotechnological processes to prepare Trichoderma-containing formulations are critically discussed.</jats:italic></jats:p><jats:p>• <jats:italic>Current challenges and future outlook of Trichoderma-based formulations in the context of biological control are presented.</jats:italic></jats:p></jats:sec>

Topics

• impedance spectroscopy
• size-exclusion chromatography
• drying