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Bonifácio, Vasco D. B.
Instituto Superior Técnico
in Cooperation with on an Cooperation-Score of 37%
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Publications (5/5 displayed)
- 2016Molecular Weight Determination by Luminescent Chemo–enzymaticscitations
- 2015Design of oligoaziridine-PEG coatings for efficient nanogold cellular biotaggingcitations
- 2015POxylated Polyurea Dendrimerscitations
- 2014Polyurea dendrimer for efficient cytosolic siRNA deliverycitations
- 2012Biocompatible polyurea dendrimers with pH-dependent fluorescencecitations
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article
Molecular Weight Determination by Luminescent Chemo–enzymatics
Abstract
<p>A novel methodology for the determination of the number-average molecular weight (M<sub>w</sub>) of linear, branched and crosslinked polymers is disclosed. The method relies on chemo-enzymatic-triggered fluorescence of dormant CdTe polymer-caged quantum dots synthesised in supercritical carbon dioxide. Polyurea (PURE) dendrimers (G1, G4, G5 and G6) are presented as a case study using the enzyme urease but the protocol is applicable to other synthetic and natural polymers. Simple, versatile and accurate, luminescent chemo-enzymatics is a low cost alternative to conventional techniques (e. g. GPC/SEC, MALDI-TOF).</p>