| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Parakhonskiy, Bogdan
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (15/15 displayed)
- 2022The influence of Ca/Mg ratio on autogelation of hydrogel biomaterials with bioceramic compoundscitations
- 2020Temperature Window for Encapsulation of an Enzyme into Thermally Shrunk, CaCO3 Templated Polyelectrolyte Multilayer Capsules.citations
- 2020Temperature window for encapsulation of an enzyme into thermally shrunk, CaCO3 templated polyelectrolyte multilayer capsulescitations
- 2020Alkaline phosphatase delivery system based on calcium carbonate carriers for acceleration of ossificationcitations
- 2020Alkaline phosphatase delivery system based on calcium carbonate carriers for acceleration of ossificationcitations
- 2019Piezoelectric 3-D fibrous poly(3-hydroxybutyrate)-based scaffolds ultrasound-mineralized with calcium carbonate for bone tissue engineering : inorganic phase formation, osteoblast cell adhesion, and proliferationcitations
- 2019The effect of hybrid coatings based on hydrogel, biopolymer and inorganic components on the corrosion behavior of titanium bone implants.citations
- 2018High-efficiency freezing-induced loading of inorganic nanoparticles and proteins into micron- and submicron-sized porous particlescitations
- 2018High-efficiency freezing-induced loading of inorganic nanoparticles and proteins into micron- and submicron-sized porous particlescitations
- 2018Composite materials based on Ag nanoparticles in situ synthesized on the vaterite porous matrices.citations
- 2018Nanostructured biointerfaces based on bioceramic calcium carbonate/hydrogel coatings on titanium with an active enzyme for stimulating osteoblasts growthcitations
- 2015Composite magnetite and protein containing CaCO3 crystals : external manipulation and vaterite → calcite recrystallization-mediated release performancecitations
- 2015Composite magnetite and protein containing CaCO3 crystalscitations
- 2009Formation of silver nanoparticles on shells of polyelectrolyte capsules using silver-mirror reactioncitations
- 2006Preparation of polyelectrolyte microcapsules with silver and gold nanoparticles in a shell and the remote destruction of microcapsules under laser irradiationcitations
Places of action
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article
Temperature Window for Encapsulation of an Enzyme into Thermally Shrunk, CaCO3 Templated Polyelectrolyte Multilayer Capsules.
Abstract
Encapsulation of enzymes allows to preserve their biological activities in various environmental conditions, such as exposure to elevated temperature or to proteases. This is particularly relevant for in vivo applications, where proteases represent a severe obstacle to maintaining the activity of enzymes. Polyelectrolyte multilayer capsules are suitable for enzyme encapsulation, where CaCO3 particles and temperature-dependent capsule formation are the best templates and the most adequate method, respectively. In this work, these two areas are combined and, ALP (alkaline phosphatase), which is a robust and therapeutically relevant enzyme, is encapsulated into thermally shrunk polyelectrolyte multilayer (PDADMAC/PSS)4 capsules templated on calcium carbonate particles (original average diameter: ≈3.5 µm). The activity of the encapsulated enzyme and the optimal temperature range for encapsulation are investigated. The enzymatic activity is almost four times higher upon encapsulation when the temperature range for encapsulation is situated just above the glass transition temperature (40 °C), while its optimal conditions are dictated, on the one hand, by the enzyme activity (better at lower temperatures) and, on the other hand, by the size and mechanical properties of capsules (better at higher temperatures).