| People | Locations | Statistics |
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| Naji, M. |
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| Motta, Antonella |
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| Aletan, Dirar |
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| Mohamed, Tarek |
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| Ertürk, Emre |
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| Taccardi, Nicola |
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| Kononenko, Denys |
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| Petrov, R. H. | Madrid |
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| Alshaaer, Mazen | Brussels |
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| Bih, L. |
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| Casati, R. |
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| Muller, Hermance |
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| Kočí, Jan | Prague |
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| Šuljagić, Marija |
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| Kalteremidou, Kalliopi-Artemi | Brussels |
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| Azam, Siraj |
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| Ospanova, Alyiya |
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| Blanpain, Bart |
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| Ali, M. A. |
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| Popa, V. |
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| Rančić, M. |
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| Ollier, Nadège |
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| Azevedo, Nuno Monteiro |
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| Landes, Michael |
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| Rignanese, Gian-Marco |
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Pinelo, Manuel
Technical University of Denmark
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2022Electrospun aluminum silicate nanofibers as novel support material for immobilization of alcohol dehydrogenasecitations
- 2022Electrospun aluminum silicate nanofibers as novel support material for immobilization of alcohol dehydrogenasecitations
- 2022Economic and environmental analysis of bio-succinic acid production: from established processes to a new continuous fermentation approach with in-situ electrolytic extractioncitations
- 2021Tailor-made novel electrospun polystyrene/poly(D,L-lactide-co-glycolide) for oxidoreductases immobilization: Improvement of catalytic properties under extreme reaction conditionscitations
- 2020Sustainable Process Synthesis, Design and Innovation of Bio-succinic Acid Productioncitations
- 2020The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removalcitations
- 2020Surface treatments and functionalization of metal‐ceramic membranes for improved enzyme immobilization performancecitations
Places of action
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article
Surface treatments and functionalization of metal‐ceramic membranes for improved enzyme immobilization performance
Abstract
Background: Enzyme immobilization in porous membranes often improves enzyme performance. This work reports the preparation and characterization of robust and scalable asymmetric metal‐ceramic microfiltration membrane. The surface of the porous metal‐ceramic membrane was treated by impregnation with a ceramic oxide for enzyme adsorption and corrosion protection. Finally, enzyme immobilization in the support was investigated.<br/><br/>Results: The bilayer membrane was successfully fabricated by combining a ceramic microfiltration layer with a metal support by tape casting, lamination and co‐sintering. A pore size in the ceramic microfiltration layer of 0.4 μm resulted in high water permeability (12000 L/(m<sup>2</sup> h bar)). Two different surface treatments were compared: heat treatment and Y<sub>2</sub>O<sub>3</sub> impregnation. Corrosion stability tests under enzyme‐relevant conditions gave no detectable chemical or structural changes. Alcohol dehydrogenase (EC 1.1.1.1) was immobilized in the membrane by physical adsorption and by two covalent immobilization methods. Covalent immobilization significantly improved enzyme loading, activity, and recyclability. Membrane reuse by heat treatment removed fouling, but decreased immobilization performance.<br/><br/>Conclusion: he improved microstructure obtained by Y<sub>2</sub>O<sub>3</sub>‐impregnation had a significant effect on enzyme loading yield and activity. This indicates the potential of this surface modification method and of these metal‐supported ceramic membranes in enzyme immobilization. Covalent immobilization was superior.