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Naji, M. |
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Motta, Antonella |
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Aletan, Dirar |
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Mohamed, Tarek |
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Ertürk, Emre |
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Taccardi, Nicola |
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Kononenko, Denys |
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Petrov, R. H. | Madrid |
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Alshaaer, Mazen | Brussels |
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Bih, L. |
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Casati, R. |
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Muller, Hermance |
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Kočí, Jan | Prague |
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Šuljagić, Marija |
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Kalteremidou, Kalliopi-Artemi | Brussels |
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Azam, Siraj |
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Ospanova, Alyiya |
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Blanpain, Bart |
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Ali, M. A. |
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Popa, V. |
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Rančić, M. |
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Ollier, Nadège |
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Azevedo, Nuno Monteiro |
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Landes, Michael |
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Rignanese, Gian-Marco |
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Yazdian, Fatemeh
in Cooperation with on an Cooperation-Score of 37%
Topics
Publications (7/7 displayed)
- 2023Chitosan/Agarose/Graphene oxide nanohydrogel as drug delivery system of 5-fluorouacil in breast cancer therapycitations
- 2023A study on the microbial biocorrosion behavior of API 5 L X65 carbon steel exposed to seawatercitations
- 2023Green synthesis of chitosan/polyacrylic acid/graphitic carbon nitride nanocarrier as a potential pH-sensitive system for curcumin delivery to MCF-7 breast cancer cellscitations
- 2023pH-responsive polyacrylic acid (PAA)-carboxymethyl cellulose (CMC) hydrogel incorporating halloysite nanotubes (HNT) for controlled curcumin deliverycitations
- 2022Construction of Aptamer-Based Nanobiosensor for Breast Cancer Biomarkers Detection Utilizing g-C3N4/Magnetic Nano-Structurecitations
- 2022Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by <i>Pseudomonas aeruginosa</i>citations
- 2021Ameliorating quercetin constraints in cancer therapy with pH-responsive agarose-polyvinylpyrrolidone -hydroxyapatite nanocomposite encapsulated in double nanoemulsioncitations
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article
Evaluation of SDS‐coated iron nanostructure on the gene expression of bio surfactant‐producing genes by <i>Pseudomonas aeruginosa</i>
Abstract
<jats:title>Abstract</jats:title><jats:p>Bio surfactants are natural surfactants that induce emulsification, displacement, increased solubility, and mobility of hydrophobic organic compounds. In this study, the gene expression of biosurfactant production genes by <jats:italic>Pseudomonas aeruginosa</jats:italic> in the presence of sodium dodecyl sulfate coated iron nanostructure (Fe/SDS) were evaluated. Emulsification Index and Surface Tension reduction test to check stability and emulsification the rhamnolipid were done. Purification was evaluated using thin layer chromatography (TLC) and expression of <jats:italic>rhlA</jats:italic>, <jats:italic>mvfR, lasR, rhlR</jats:italic> genes was determined using q‐PCR technique. Binding of nanoparticles to bio surfactants was confirmed by TEM. The best emulsification index, was by the sample that exposed to 1 mg/L Fe/SDS nanoparticles for 2 days. Rhamnolipid produced in the presence of nanoparticles had an acceptable ability to reduce surface tension. The Rf (retention factor) value obtained was 0.63 by chromatography. q‐PCR results showed that the expression <jats:italic>of rhlA, mvfR, lasR, rhlR</jats:italic> genes was significantly increased in Fe/SDS treated cells, which indicates the significant positive effect (<jats:italic>P</jats:italic> < 0.05) of nanoparticles on biosurfactant production of treated cells. While, SDS and Fe alone were not affected significantly (<jats:italic>P</jats:italic> > 0.05) on the expression of these genes. Our findings indicated the importance of nanoparticles in increasing the expression of genes involved in the bio surfactant production pathway of <jats:italic>Pseudomonas aeruginosa</jats:italic>.</jats:p>